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Development of competitive and noncompetitive lateral flow immunoassays for pendimethalin using synthetic peptides

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Abstract

Peptidomimetic and anti-immunocomplex peptides can be easily isolated from phage display libraries, and can be used as alternatives to chemical competing haptens to improve the sensitivity of small molecule immunoassay. In this work, 16 peptidomimetics and 7 anti-immunocomplex peptides of pendimethalin (PND) were obtained from cyclic 7-, 8-, 9-, and 10-residue peptide phage libraries. Peptidomimetic EJ-2 (CMFTGTDFPC) with the highest sensitivity in competitive phage enzyme-linked immunosorbent assay (ELISA) and immunocomplex peptide EF-30 (CNPGWPPIPC) with the highest sensitivity in noncompetitive phage ELISA were selected to prepare phage-free peptides with GGGSSK-biotin at the C-terminus. Competitive and noncompetitive lateral flow immunoassays (CLFIA and NLFIA) were developed by using the phage-free peptides. After optimization, the CLFIA and NLFIA showed visual limit of detections (vLODs) of 5 ng/mL and 2.5 ng/mL, respectively, which were improved two- and fourfold compared with a LFIA based on chemical hapten. The NLFIA showed better sensitivity than CLFIA in the detection of spiked samples, and can meet the detection requirements for agro-products regulated by EU and China. The detection results of CLFIA and NLFIA for blind samples were consistent with that of ultra performance liquid chromatography/tandem mass spectrometry.

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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by Jiangsu Agriculture Science and Technology Innovation Fund (Grant No. CX(22)3075) and Inner Mongolia Science & Technology Plan [Grant No. 2022YFSH0050].

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Authors and Affiliations

  1. College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China

    Lianrun Huang, Guixiu Wang, Yanling Wu, Zhijia Wang, Yuan Ding & Xiude Hua

  2. State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing, 210095, China

    Lianrun Huang, Guixiu Wang, Yanling Wu, Zhijia Wang, Yuan Ding & Xiude Hua

  3. Inner Mongolia Key Laboratory of Environmental Pollution Control & Waste Resource Reuse, School of Ecology and Environment, Inner Mongolia University, Hohhot, 010021, China

    Hongwu Liang

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  1. Lianrun Huang
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Huang, L., Wang, G., Wu, Y. et al. Development of competitive and noncompetitive lateral flow immunoassays for pendimethalin using synthetic peptides. Microchim Acta 191, 68 (2024). https://doi.org/10.1007/s00604-023-06151-w

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