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Two-photon fluorescence probe for quantification of cyclosporine

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Abstract

A novel ratiometric fluorescent sensor was fabricated for the fast and facile determination of cyclosporine A (CsA). Due to the narrow therapeutic index of CsA, its desired therapeutic effects are evident within a limited range of blood concentration, indicating the fundamental role of therapeutic drug monitoring in CsA pharmacological response. In this study, a two-photon fluorescence probe based on the zeolitic imidazolate framework (ZIF-8) and norepinephrine-capped silver nanoparticles (AgNPs@NE) was employed for the quantification of the CsA in human plasma samples. In the presence of CsA, the fluorescent emission intensity of ZIF-8-AgNPs@NE was quenched. Under the optimum conditions, the proposed probe determines CsA in plasma samples in two linear ranges of 0.01 to 0.5 μg mL−1 and 0.5 to 10 μg mL−1. The developed probe demonstrates the advantages of a facile and fast platform with limit of detection as low as 0.007 μg mL−1. At last, this method was applied to find CsA concentration in four patients receiving oral CsA regimen which indicates it as a promising method for on-site detection applications.

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Funding

This study was supported financially by Tabriz University of Medical Sciences with registration number of 68609.

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Authors and Affiliations

  1. Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 51656-65811, Iran

    Zahra Golsanamlu, Jafar Soleymani & Abolghasem Jouyban

  2. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, 51664-14766, Iran

    Zahra Golsanamlu

  3. Department of Clinical Pharmacy (Pharmacotherapy), Faculty of Pharmacy, Sina Hospital, Tabriz University of Medical Sciences, Tabriz, 51636-39889, Iran

    Afshin Gharekhani

  4. Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, 19857-17443, Iran

    Abolghasem Jouyban

Authors
  1. Zahra Golsanamlu
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Correspondence to Jafar Soleymani.

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Golsanamlu, Z., Soleymani, J., Gharekhani, A. et al. Two-photon fluorescence probe for quantification of cyclosporine. Microchim Acta 190, 266 (2023). https://doi.org/10.1007/s00604-023-05852-6

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