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Mn3O4 nanoparticles decorated porous reduced graphene oxide with excellent oxidase-like activity for fast colorimetric detection of ascorbic acid

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Abstract

Mn3O4 nanoparticles composed of porous reduced graphene oxide nanosheets (Mn3O4@p-rGO) with enhanced oxidase-like activity were successfully fabricated through an in-situ approach for fast colorimetric detection of ascorbic acid (AA). The residual Mn2+ in the GO suspension of Hummers method was directly reused as the manganese source, improving the atom utilization efficiency. Benefiting from the uniform distribution of Mn3O4 nanoparticles on the surface of p-rGO nanosheets, the nanocomposite exhibited larger surface area, more active sites, and accelerated electron transfer efficiency, which enhanced the oxidase-like activity. Mn3O4@p-rGO nanocomposite efficiently activate dissolved O2 to generate singlet oxygen (1O2), leading to high oxidation capacity toward the substrate 3,3′,5,5′–tetramethylbenzidine (TMB) without the extra addition of H2O2. Furthermore, the prominent absorption peak of the blue ox-TMB at 652 nm gradually decreased in the presence of AA, and a facile and fast colorimetric sensor was constructed with a good linear relationship (0.5–80 μM) and low LOD (0.278 μM) toward AA. Owing to the simplicity and excellent stability of the sensing platform, its practical application for AA detection in juices has shown good feasibility and reliability compared with HPLC and the 2, 4-dinitrophenylhydrazine colorimetric method. The oxidase-like Mn3O4@p-rGO provides a versatile platform for applications in food testing and disease diagnosis.

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Funding

The work was supported by the National Natural Science Foundation of China (21964003), the Science and Technology Project of the Education Department of Jiangxi Province (490042), the Double Thousand Talents Program of Jiangxi Province (jxsq2019102009), and the College Students’ Innovation and Entrepreneurship Projects of Gannan Normal University (CX220058).

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  1. Dong Peng and Mingming Que contributed equally to this work.

Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Gannan Normal University, Ganzhou, 341000, China

    Dong Peng, Mingming Que, Xiulong Deng, Qifang He, Yuhong Zhao, Shuzhen Liao, Xun Li & Hongdeng Qiu

  2. CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, China

    Hongdeng Qiu

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  1. Dong Peng
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Peng, D., Que, M., Deng, X. et al. Mn3O4 nanoparticles decorated porous reduced graphene oxide with excellent oxidase-like activity for fast colorimetric detection of ascorbic acid. Microchim Acta 190, 243 (2023). https://doi.org/10.1007/s00604-023-05822-y

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