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Co(OH)2/MXene-Ti3C2 nanocomposites with triple-enzyme mimic activities as hydrogel sensing platform for on-site detection of hypoxanthine

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Abstract 

Novel Co(OH)2/MXene-Ti3C2 nanocomposites with oxidase (OXD)-mimic, peroxidase (POD)-mimic, and catalase (CAT)-mimic activities were prepared by a simple two-step method. The Co(OH)2/MXene-Ti3C2 nanocomposites with triple-enzyme mimic activities were embedded into sodium alginate (SA) gels for the first time to fabricate a target-responsive hydrogel-assisted assay. The catalytic mechanism and steady-state kinetics of Co(OH)2/MXene-Ti3C2 nanocomposites were investigated. Subsequently, hypoxanthine (Hx) was catalyzed by xanthine oxidase (XOD) to form H2O2, which reacts with 3,3′,5,5′-tetramethyl-benzidine (TMB) in the presence of Co(OH)2/MXene-Ti3C2 nanocomposites to form a blue oxide (ox-TMB) in the hydrogel. The visible color change of the hydrogel with the increase of Hx concentration can be recognized through a smartphone App to transfer the red (R), green (G), and blue (B) values for the quantitative determination of  Hx, with a detection range from 5 to 250 μM, and detection limit of 0.2 μM. The method was applied to the determination of Hx content in different aquatic products. The spiked recoveries of the aquatic products were from 94.1 to 106.4%, and the relative standard deviations (RSD) were less than 5.4%. Our results show that the Co(OH)2/MXene-Ti3C2 nanocomposites hydrogel-assisted colorimetric biosensor is cost-effective, sensitive, and selective and has excellent application prospects for in-the-field determination of Hx.

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Funding

This work was financially supported by the National Natural Science Foundation of China (21707030 and 82073608).

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Authors and Affiliations

  1. Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, China

    Qinqin Yang, Ying Qin, Tiantian Lei, Xipeng Cui, Yu He & Gongwu Song

  2. Key Laboratory of Textile Fiber and Products (Wuhan Textile University), Ministry of Education, Hubei International Scientific and Technological Cooperation Base of Intelligent Textile Materials & Application, Wuhan Textile University, Wuhan, 430200, China

    Yaping Zhong

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  1. Qinqin Yang
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  2. Ying Qin
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Yang, Q., Qin, Y., Lei, T. et al. Co(OH)2/MXene-Ti3C2 nanocomposites with triple-enzyme mimic activities as hydrogel sensing platform for on-site detection of hypoxanthine. Microchim Acta 189, 481 (2022). https://doi.org/10.1007/s00604-022-05584-z

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