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Iron porphyrin-based porous organic polymer with high peroxidase-like activity as colorimetric sensor for glutathione and ascorbic acid assay

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Abstract

A new iron porphyrin-based organic polymer (Fe-POP) was synthesized through the William ether reaction. The as-prepared Fe-POP presented high chemical stability, wide pore distribution, high iron content, and strong affinity with 3,3’,5,5’-tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2), which contributed to its excellent peroxidase-mimicking performance. In the presence of H2O2, Fe-POP could catalyze the transparent TMB into blue ox-TMB, which could be easily distinguished by the naked eyes. Moreover, glutathione (GSH) and ascorbic acid (AA) could convert blue ox-TMB into colorless TMB due to the inhibitory effect of GSH/AA to the catalytic oxidation of TMB. Based on this phenomenon, a rapid and sensitive colorimetric method for the assay of H2O2, GSH, and AA was developed using Fe-POP as sensor. The detection limits of H2O2, GSH, and AA  were 1.37, 0.44, and 0.33 μM, respectively. Finally, the colorimetric method based on Fe-POP was used to evaluate the GSH and AA content in real samples, which provided the guidance for GSH and AA supplements in our daily diet, suggesting the significant potential of Fe-POP in practical applications.

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Funding

This work was supported by National Natural Science Foundation of China (NSFC) (grant no. 21601142), Outstanding Young and Middle-aged Scientific Innovation Team of Colleges and Universities of Hubei Province: “Biomass chemical technologies and materials” (grant no. T201908), the Innovation Project of Key Laboratory of Novel Biomass-Based Environmental and Energy Materials in Petroleum and Chemical Industry (grant no. 2022BEEA06).

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Authors and Affiliations

  1. Key Laboratory of Novel Biomass-Based Environmental and Energy Materials in Petroleum and Chemical Industry, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan, 430072, People’s Republic of China

    Linhong Liao, Simiao Tong, Xiaogang Luo, Genyan Liu & Fengshou Wu

  2. Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan, 430072, People’s Republic of China

    Simiao Tong, Genyan Liu & Fengshou Wu

  3. School of Materials Science and Engineering, Zhengzhou University, Zhengzhou, 450001, People’s Republic of China

    Xiaogang Luo

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  1. Linhong Liao
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Correspondence to Genyan Liu or Fengshou Wu.

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Liao, L., Tong, S., Luo, X. et al. Iron porphyrin-based porous organic polymer with high peroxidase-like activity as colorimetric sensor for glutathione and ascorbic acid assay. Microchim Acta 189, 384 (2022). https://doi.org/10.1007/s00604-022-05471-7

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