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Aptamer-modified magnetic SERS substrate for label-based determination of cardiac troponin I

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Abstract

A sensitive label-based SERS strategy composed of magnetic bimetallic nanoparticles Fe3O4@Ag@Au, specific aptamer, and Bradford method was developed for the quantitative determination of cardiac troponin I (cTnI) in human serum. The prepared substrate with high magnetic character, signal enhancement, and uniformity exhibited significant Raman response. After the substrate was bound to the aptamer, the target protein cTnI was specifically captured, and it showed the Raman signal when the signal reporter Coomassie Brilliant Blue G-250 (CBBG) was supplied. The Raman signal intensity at 1621 cm−1 showed a wide linear relationship with the log value of the cTnI concentration in the range 0.01 to 100 ng·mL−1, and the estimated limit of detection (LOD) was 5.50 pg·mL−1. The recovery and relative standard deviation (RSD) of the spike experiment in human serum samples were 92–115% and 7.4–12.7%, respectively.

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Funding

This work was financially supported by National Natural Science Foundation of China (Grant No. 81860633) and the Natural Science Foundation of Guangxi (Grant No. 2020GXNSFAA 297168, 2016GXNSFAA380108).

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Authors and Affiliations

  1. Guangxi Key Laboratory of Green Processing of Sugar Resources, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, No.268 Donghuan Road, Chengzhong District, Liuzhou City, 545006, Guangxi Zhuang Autonomous Region, China

    Chubing Lin, Lijun Li, Jun Feng, Yan Zhang, Xin Lin, Heyuanxi Guo & Rui Li

  2. Province and Ministry Co-Sponsored Collaborative Innovation Center of Sugarcane and Sugar Industry, Nanning, 530004, Guangxi Zhuang Autonomous Region, China

    Chubing Lin, Lijun Li, Jun Feng, Yan Zhang, Xin Lin, Heyuanxi Guo & Rui Li

Authors
  1. Chubing Lin
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  2. Lijun Li
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  3. Jun Feng
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  4. Yan Zhang
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  5. Xin Lin
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  6. Heyuanxi Guo
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  7. Rui Li
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Contributions

Conceptualization, CB Lin, LJ Li, J Feng, Y Zhang, X Lin, HYX Guo, and R Li; writing (original draft preparation), CB Lin and LJ Li; writing (review and editing), CB Lin, LJ Li, J Feng, Y Zhang, X Lin, HYX Guo, and R Li. Funding acquisition, LJ Li.

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Correspondence to Lijun Li.

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Lin, C., Li, L., Feng, J. et al. Aptamer-modified magnetic SERS substrate for label-based determination of cardiac troponin I. Microchim Acta 189, 22 (2022). https://doi.org/10.1007/s00604-021-05121-4

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