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Fluorescent hollow ZrO2@CdTe nanoparticles-based lateral flow assay for simultaneous detection of C-reactive protein and troponin T

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Abstract

Highly fluorescent hollow ZrO2@CdTe nanoparticles (NPs) were synthesized efficiently via the hydrothermal method. By changing the hydrothermal time of ZrO2@CdTe NP, the peaks of fluorescence spectra measured at fluorescent excitation of 330 nm were at 540 nm, 590 nm, and 640 nm, respectively. Hollow ZrO2 NPs have a uniform core–shell structure with the size of 178 ± 10 nm and shell of 19 ± 4 nm. The as-prepared yellow-ZrO2@CdTe NPs were used to develop lateral flow assay (LFA) for the sensitive and qualitative detection of C-reactive protein (CRP). The visual limit of detection of the LFA for the CRP antigen was 1 μg/L within 20 min, which is 1000-fold lower than that of colloidal gold-based LFA. In addition, a multiplex lateral flow assay (mLFA) was developed using the as-prepared green and red-ZrO2@CdTe NPs for the simultaneous, specific, sensitive, and qualitative detection of CRP and troponin T (cTnT). The visual limits of detection of CRP and cTnT in mLFA were 10 μg/L and 0.1 mg/L, respectively. The excellent performance of ZrO2@CdTe NPs should facilitate their application in point-of-care technology for the detection of other biomarkers.

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Acknowledgements

We acknowledge the financial support from the Beijing Natural Science Foundation (4202075, 2194087, and 4161003), National Natural Science Foundation of China (61975214, 81630053, and 61571426) and Scientific and Technologial Innovation Programs of Higher Education Institutions in Shanxi (2020L0199).

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Authors and Affiliations

  1. Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China

    Xin Liu, Xiangling Ren, Jian Zou, Ting Li, Longfei Tan, Changhui Fu, Qiong Wu & Xianwei Meng

  2. College of Materials Science & Engineering, Sichuan University, Chengdu, 610065, China

    Xin Liu, Jian Zou & Zhongbing Huang

  3. CAS Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China

    Xin Liu, Xiangling Ren, Jian Zou, Ting Li, Longfei Tan, Changhui Fu, Qiong Wu & Xianwei Meng

  4. University of Chinese Academy of Sciences, Beijing, 100049, China

    Xiangling Ren & Ting Li

  5. First Clinical Medical School and First Hospital, Shanxi Medical University, 030001, Taiyuan, China

    Lufeng Chen

  6. Beijing Zhongsheng Jinyu Diagnostic Technology Corporation Co. Ltd, Beijing, 102200, China

    Chaohui Li & Jiayi Wang

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  1. Xin Liu
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Liu, X., Ren, X., Chen, L. et al. Fluorescent hollow ZrO2@CdTe nanoparticles-based lateral flow assay for simultaneous detection of C-reactive protein and troponin T. Microchim Acta 188, 209 (2021). https://doi.org/10.1007/s00604-021-04865-3

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