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Highly porous terpolymer-ZIF8@BA MOF composite for identification of mono- and multi-glycosylated peptides/proteins using MS-based bottom-up approach

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Abstract

A hydrophilic terpolymer MOF composite is designed with high surface area and porosity to enrich mono- and multi-glycosylated peptides facilitating a bottom-up approach. Terpolymer@ZIF-8 is synthesized using free radical polymerization followed by layer by layer ZIF-8 fabrication. Subsequent surface modification was made by aminophenylboronic acid (AMBA). The enrichment ability of terpolymer@ZIF-8@BA is evaluated by using tryptic digest of IgG and HRP to exemplify mono- and multi-glycosylated protein samples. Improved selectivity of 1:200 for spiked HRP in BSA digest and sensitivity down to 1 fmol μL−1 is achieved. Batch to batch reproducibility is better 1% RSD which favors the adoption of the developed method for routine N-linked glycopeptide/protein determination. Cost-effective nature of given approach is given by regeneration of the material up to four cycles. Total 318 N-linked glycopeptides have been identified from 1 μL human serum digest after subjecting the enriched and PNGase-treated deglycosylated peptides to LC-MS. Thus, terpolymer@ZIF-8@BA holds the potential both for mono- and multi-glycosylated peptides from complex biological sample.

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Funding

This work was supported by Higher Education Commission (HEC) of Pakistan.

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Authors and Affiliations

  1. Department of Chemistry, The Women University, Kutchery Campus, L.M.Q. Road, Multan, 66000, Pakistan

    Shafaq Saleem, Fahmida Jabeen & Adeela Saeed

  2. Division of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, 60800, Pakistan

    Muhammad Salman Sajid, Dilshad Hussain, Fahmida Jabeen, Muhammad Najam-ul-Haq & Adeela Saeed

  3. International Centre for Chemical and Biological Sciences, HEJ Research Institute of Chemistry, University of Karachi, Karachi, 75270, Pakistan

    Dilshad Hussain

  4. Department of Biochemistry, Bahauddin Zakariya University, Multan, 60800, Pakistan

    Batool Fatima

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  1. Shafaq Saleem
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Correspondence to Adeela Saeed.

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Serum samples were obtained from anonymous healthy males from the local hospital in accordance with government regulations and approved by the Ethical Advisory Board, Pakistan.

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Saleem, S., Sajid, M.S., Hussain, D. et al. Highly porous terpolymer-ZIF8@BA MOF composite for identification of mono- and multi-glycosylated peptides/proteins using MS-based bottom-up approach. Microchim Acta 187, 555 (2020). https://doi.org/10.1007/s00604-020-04532-z

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