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A nanosorbent consisting of a magnetic molecularly imprinted polymer and graphene oxide for multi-residue analysis of cephalosporins

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Abstract

A nanosorbent composed of magnetite nanoparticles, graphene oxide and a molecularly imprinted polymer (Fe3O4@SiO2-NH2/GOx/MIP) was synthesized and applied to simultaneous extraction of cephalexin, cefazolin and cefoperazone from milk. The use of magnetite nanoparticles enables fast extraction by using an external magnet. The use of graphene oxide increases extraction affinity, and the MIP improves selectivity. Extraction efficiency was optimized by investigating the effects of the template-to-monomer and cross-linker ratios, the desorption condition, extraction time, salting-out effect, stirring rate, sample volume and amount of adsorbent. The cephalosporins were quantified by using HPLC. Under optimum condition, the linear range of the method extends from 2.5 to 100 μg L−1 for cephalexin and cefazolin, and from 5.0 to 100 μg L−1 for cefoperazone. The limits of detection are 2.5 μg L−1 for cephalexin and cefazolin, and 5 μg L−1 for cefoperazone. The adsorbent was applied to the extraction of cephalosporins from milk, with recoveries in a range from 80.2 to 111.7% and with RSDs of <8.5%.

Schematic representation of a nanocomposite adsorbent consisting of magnetic molecularly imprinted polymer and graphene oxide (GOx). Integrating of magnetite nanoparticles, GOx and high specificity of MIP, the method exhibited a rapid, high extraction efficiency, good selectivity for multi-residue analysis of cephalosporins.

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Acknowledgments

This work was supported by the Thailand Research Fund (MRG6180049), the Higher Education Research Promotion and the Thailand’s Education Hub for Southern Region of ASEAN Countries Project Office of the Higher Education Commission, the Center of Excellence for Innovation in Chemistry (PERCH-CIC), Ministry of Higher Education, Science, Research and Innovation and Prince of Songkla University, Thailand (Grant No. SCI6202115N-0).

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  1. Center of Excellence for Innovation in Chemistry, Department of Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Miftah Nurrokhimah, Piyaluk Nurerk, Proespichaya Kanatharana & Opas Bunkoed

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  1. Miftah Nurrokhimah
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  3. Proespichaya Kanatharana
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Correspondence to Opas Bunkoed.

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Nurrokhimah, M., Nurerk, P., Kanatharana, P. et al. A nanosorbent consisting of a magnetic molecularly imprinted polymer and graphene oxide for multi-residue analysis of cephalosporins. Microchim Acta 186, 822 (2019). https://doi.org/10.1007/s00604-019-3985-5

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