Abstract
A composite nanoenzyme was used in a sandwich-type electrochemical immunoassay for the carcinoembryonic antigen (CEA). Hierarchically porous palladium nanospheres (Pd NPs) were functionalized with glutathione-capped gold nanoparticles (G-Au NPs) and then loaded onto graphene oxide (GO) to obtain a peroxidase mimicking nanoenzyme of type GO-supported G-Au/Pd. The composite can catalyze the oxidation of the substrate tetramethylbenzidine (TMB) by H2O2 to give blue-colored oxidized TMB within only 20 s. This strong peroxidase activity, good conductivity and high specific surface area of the material make it a useful label for secondary antibodies (Ab2) for the detection of CEA. The cotton-like electrodeposited gold nanoparticles with good electrical conductivity were used to immobilize primary antibody (Ab1). The amperometric immunoassay has a detection range that extends from 10 fg·mL−1 to 100 ng·mL−1 at a working potential of −0.4 V with addition of 5 mmol·L−1 H2O2 as electrochemically active substrate, and the detection limit is as low as 3.2 fg·mL−1 (S/N = 3).
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Acknowledgements
We greatly appreciate the support of the National Natural Science Foundation of China (21405095; 21575079; 21671121) and the Shandong Provincial Natural Science Foundation (ZR2018MB008, ZR2018MB012).
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Tan, Z., Cao, L., Yang, Y. et al. Amperometric immunoassay for the carcinoembryonic antigen by using a peroxidase mimic consisting of palladium nanospheres functionalized with glutathione-capped gold nanoparticles on graphene oxide. Microchim Acta 186, 693 (2019). https://doi.org/10.1007/s00604-019-3799-5
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DOI: https://doi.org/10.1007/s00604-019-3799-5