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Colorimetric tyrosinase assay based on catechol inhibition of the oxidase-mimicking activity of chitosan-stabilized platinum nanoparticles

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Abstract

It is found that catechol inhibits the oxidase-mimicking activity of chitosan-protected platinum nanoparticles (Chit-PtNPs) by competing with the substrate for the active site of the Ch-PtNPs. The inhibition mechanism of catechol is different from that of ascorbic acid in that it neither reacts with O2•- nor reduces the oxidized 3,3′,5,5′-tetramethylbenzidine (TMB). Tyrosinase (TYRase) catalyzes the oxidation of catechol, thus restoring the activity of oxidase-mimicking Chit-PtNPs. By combining the Chit-PtNP, catechol, and TYRase interactions with the oxidation of TMB to form a yellow diamine (maximal absorbance at 450 nm), a colorimetric analytical method was developed for TYRase determination and inhibitor screening. The assay works in the 0.5 to 2.5 U·mL−1 TYRase activity range, and the limit of detection is 0.5 U·mL−1. In our perception, this new assay represents a powerful approach for determination of TYRase activity in biological samples.

Schematic representation of a colorimetric method for tyrosinase (TYRase) detection and inhibitor screening. It is based on the fact that catechol can inhibit the oxidase-like activity of chitosan-stabilized platinum nanoparticles (Ch-PtNPs) by competing with the substrate for the active sites and TYRase can catalyze the oxidation of catechol.

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Acknowledgements

The authors gratefully acknowledge the financial support from the National Natural Science Foundation of China (21675024, 21804021), the Program for Innovative Leading Talents in Fujian Province (2016B016), the Science and Technology Project of Fujian Province (2018 L3008), Natural Science Foundation of Fujian Province (2016 J01427, 2016 J06019), and Startup Fund for scientific research, Fujian Medical University (2017XQ1014).

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Author notes

  1. Hao-Hua Deng and Xiu-Ling Lin contributed equally to this work.

Authors and Affiliations

  1. Nano Medical Technology Research Institute, Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350004, China

    Hao-Hua Deng, Xiu-Ling Lin, Shao-Bin He, Gang-Wei Wu, Wei-Hua Wu, Yu Yang, Zhen Lin, Hua-Ping Peng & Wei Chen

  2. Department of Pharmacy, Fujian Provincial Hospital, Fuzhou, 350001, China

    Gang-Wei Wu

  3. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China

    Xing-Hua Xia

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  1. Hao-Hua Deng
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Correspondence to Hua-Ping Peng or Wei Chen.

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Deng, HH., Lin, XL., He, SB. et al. Colorimetric tyrosinase assay based on catechol inhibition of the oxidase-mimicking activity of chitosan-stabilized platinum nanoparticles. Microchim Acta 186, 301 (2019). https://doi.org/10.1007/s00604-019-3451-4

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