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A bifunctional metal organic framework of type Fe(III)-BTC for cascade (enzymatic and enzyme-mimicking) colorimetric determination of glucose

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Abstract

A metal organic framework (MOF) of type Fe(III)-BTC (where BTC is 1,3,5-benzenetricarboxylic acid) was utilized to construct an integrated system for cascade colorimetric determination of glucose. The MOF performs a dual function in acting (a) as a peroxidase (POx) mimic, and (b) as a solid support for immobilization of glucose oxidase (GOx). The MOF was prepared by a one-pot method. Glucose is consumed while H2O2 is produced during the enzymatic oxidation by GOx. In the presence of H2O2, the POx mimic catalytically oxidizes 3,3′,5,5′-tetramethylbenzidine (TMB) to form a blue-green product. The absorbance of oxidized TMB (measured at 652 nm) increases linearly in the 5.0–100 μM glucose concentration range, and the detection limit is 2.4 μM. The GOx@Fe-BTC MOF was successfully applied to the determination of glucose in serum.

Schematic presentation of a bifunctional metal organic framework of type Fe-BTC for cascade (enzymatic and enzyme-mimicking) colorimetric determination of glucose. The Fe-BTC performs a dual function in acting as both a peroxidase mimic and support for immobilizing glucose oxidase. Using the integrated enzyme, a colorimetric method was successfully applied to one-step detection of glucose in human serum.

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Acknowledgements

The financial support from the National Natural Science Foundation of China (21765002), Guangxi Natural Science Foundation of China (2017GXNSFDA198044), State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University) (CMEMR2017-A10, CMEMR2018-C11), and the BAGUI Scholar Program is gratefully acknowledged.

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Authors and Affiliations

  1. State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Pharmaceutical Science of Guangxi Normal University, Guilin, 541004, People’s Republic of China

    Zhenghong Zhao, Junhong Pang, Wenren Liu, Tianran Lin, Fanggui Ye & Shulin Zhao

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  1. Zhenghong Zhao
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  2. Junhong Pang
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  3. Wenren Liu
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  4. Tianran Lin
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  5. Fanggui Ye
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Correspondence to Fanggui Ye.

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Zhao, Z., Pang, J., Liu, W. et al. A bifunctional metal organic framework of type Fe(III)-BTC for cascade (enzymatic and enzyme-mimicking) colorimetric determination of glucose. Microchim Acta 186, 295 (2019). https://doi.org/10.1007/s00604-019-3416-7

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