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Cobalt oxyhydroxide modified with poly-β-cyclodextrin and a cyanine dye as a nanoplatform for two-photon imaging of ascorbic acid in living cells and tissue

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Abstract

This article describes the development of several nanoconjugates composed of cobalt (III) oxyhydroxide and DEASPI/βCDP, where DEASPI stands for the dye trans-4-[p-(N,N-diethylamino)styryl]-N-methylpyridinium, and βCDP stands for β-cyclodextrin. The material enables sensitive fluorometric detection and 3D imaging of ascorbic acid (AA) in biological samples. A nanomicelle composed of DEASPI and βCDP was prepared to act as a two-photon absorbance (TPA) nanofluorophore with desirable two-photon-sensitized fluorescence, high penetration depth, and excellent cell-permeability). The CoOOH nanoflakes were placed on the surface of the nanomicelle to act as both a quencher of fluorescence and as the recognition unit for AA. In the presence of AA, the CoOOH nanoflakes are reduced to Co (II), and this triggers the recovery of fluorescence. This new nanoprobe exhibits amplified two-photon fluorescence (excitation at 840 nm; emission at 565 nm), high sensitivity, and good selectivity. In-vitro imaging of endogenous AA was demonstrated in living HeLa cells. It was also employed to 3D imaging of exogenous AA in tissue by two-photon excitation microscopy to a depth of up to 320 μm. In our perception, this nanoprobe represents a valuable tool for elucidating the roles of AA in biochemical and clinical studies.

Schematic presentation of the preparation of a novel Poly β-Cyclodextrin/TPdye conjugated with cobalt oxyhydroxide nanoplatform and its application for high sensitive and two-photon 3D imaging of ascorbic acid (AA) in living cells and deep tissues.

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Acknowledgements

We are grateful for the financial support from the National Natural Science Foundation of China (No. 21605127), Doctorial Starting Fund of Xinxiang Medical University, Key Research Projects of Henan Higher Education Institutions (No. 19A416007), and Xinxiang Innovative Technology Team (No. CXTD17004).

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Authors and Affiliations

  1. School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan, 453003, People’s Republic of China

    Huijuan Yan, Yufei Liu, Jingfang Shangguan & Xue Yang

  2. School of Biomedical Engineering, Xinxiang Neurosense and Control Engineering Technology Research Center, Xinxiang Key Lab of Biomedical Information Research, Xinxiang Medical University, Xinxiang, Henan, 453003, People’s Republic of China

    Wu Ren

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  1. Huijuan Yan
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Yan, H., Liu, Y., Ren, W. et al. Cobalt oxyhydroxide modified with poly-β-cyclodextrin and a cyanine dye as a nanoplatform for two-photon imaging of ascorbic acid in living cells and tissue. Microchim Acta 186, 201 (2019). https://doi.org/10.1007/s00604-019-3320-1

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