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Silicon quantum dot-coated onto gold nanoparticles as an optical probe for colorimetric and fluorometric determination of cysteine

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Abstract

Silicon quantum dots (SiQDs) were synthesized from N-[3-(trimethoxysilyl)propyl]-ethylenediamine and catechol by a hydrothermal method. Transmission electron microscopy, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy were used to characterize the morphology and structure of quantum dots. The SiQDs were then placed on gold nanoparticles (AuNPs). When Cys is added to this solution, Cys will penetrate the SiQDs “shell” of the SiQDs/AuNP composite. This is due to the interaction and conformational differences of Cys and other substance with AuNPs and leads to the dispersion of the aggregated SiQD/AuNPs. A color change from purple to red can be visually observed, and the (green) fluorescence of SiQDs (with excitation/emission peaks at 430/520 nm) is restored. This dual-readout nanoprobe was successfully applied to the selective and sensitive detection of cysteine (Cys) in (spiked) serum and urine samples. The detection limit is 3.5 nmol·L−1 (at an S/N ratio of 3), and the method works on the 0.01 to 2 μM Cys concentration range.

Schematic illustration of a method for synthesizing silicon quantum dots (SiQDs) and coating them on gold nanoparticles (AuNPs) as an optical probe for colorimetric and fluorometric determination of cysteine.

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References

  1. Chen C, Zhou L, Liu W, Liu W (2018) Coumarinocoumarin-based two-photon fluorescent cysteine biosensor for targeting lysosome. Anal Chem 90:6138–6143

    Article  CAS  Google Scholar 

  2. Kong F, Liu R, Chu R, Wang X, Xu K, Tang B (2013) A highly sensitive near-infrared fluorescent probe for cysteine and homocysteine in living cells. Chem Commun 49:9176–9178

    Article  CAS  Google Scholar 

  3. Inoue T, Kirchhoff JR (2002) Determination of thiols by capillary electrophoresis with amperometric detection at a coenzyme pyrroloquinoline quinone modified electrode. Anal Chem 74:1349–1354

    Article  CAS  Google Scholar 

  4. Zhang W, Li P, Geng Q, Duan Y, Guo M, Cao Y (2014) Simultaneous determination of glutathione, cysteine, homocysteine, and cysteinylglycine in biological fluids by ion-pairing high-performance liquid chromatography coupled with precolumn derivatization. J Agric Food Chem 62:5845–5852

    Article  CAS  Google Scholar 

  5. Ganganboina AB, Dutta Chowdhury A, Doong RA (2018) N-doped graphene quantum dots-decorated V2O5 nanosheet for fluorescence turn off-on detection of cysteine. ACS Appl Mater Interfaces 10:614–624

    Article  CAS  Google Scholar 

  6. Chen X, Zhou Y, Peng X, Yoon J (2010) Fluorescent and colorimetric probes for detection of thiols. Chem Soc Rev 39:2120–2135

    Article  CAS  Google Scholar 

  7. Tang Z, Lin Z, Li G, Hu Y (2017) Amino nitrogen quantum dots-based nanoprobe for fluorescence detection and imaging of cysteine in biological samples. Anal Chem 89:4238–4245

    Article  CAS  Google Scholar 

  8. Deng J, Lu Q, Hou Y, Liu M, Li H, Zhang Y, Yao S (2015) Nanosensor composed of nitrogen-doped carbon dots and gold nanoparticles for highly selective detection of cysteine with multiple signals. Anal Chem 87:2195–2203

    Article  CAS  Google Scholar 

  9. Lim SY, Hong KH, Kim DI, Kwon H, Kim HJ (2014) Tunable heptamethine-azo dye conjugate as an NIR fluorescent probe for the selective detection of mitochondrial glutathione over cysteine and homocysteine. J Am Chem Soc 136:7018–7025

    Article  CAS  Google Scholar 

  10. Yin GX, Niu TT, Gan YB, Yu T, Yin P, Chen HM, Zhang YY, Li HT, Yao SZ (2018) A multi-signal fluorescent probe with multiple binding sites for simultaneous sensing of cysteine, homocysteine, and glutathione. Angew Chem Int Ed Eng 57:4991–4994

    Article  CAS  Google Scholar 

  11. Chen Q, Liu M, Zhao J, Peng X, Chen X, Mi N, Yin B, Li H, Zhang Y, Yao S (2014) Water-dispersible silicon dots as a peroxidase mimetic for the highly-sensitive colorimetric detection of glucose. Chem Commun 50:6771–6774

    Article  CAS  Google Scholar 

  12. Zhang J, Yu SH (2014) Highly photoluminescent silicon nanocrystals for rapid, label-free and recyclable detection of mercuric ions. Nanoscale 6:4096–4101

    Article  CAS  Google Scholar 

  13. Zhang XD, Chen XK, Yang JJ, Jia HR, Li YH, Chen Z, Wu FG (2016) Quaternized silicon nanoparticles with polarity-sensitive fluorescence for selectively imaging and killing gram-positive bacteria. Adv Funct Mater 26:5958–5970

    Article  CAS  Google Scholar 

  14. Zhong Y, Peng F, Bao F, Wang S, Ji X, Yang L, Su Y, Lee ST, He Y (2013) Large-scale aqueous synthesis of fluorescent and biocompatible silicon nanoparticles and their use as highly photostable biological probes. J Am Chem Soc 135:8350–8356

    Article  CAS  Google Scholar 

  15. Zhong Y, Sun X, Wang S, Peng F, Bao F, Su Y, Li Y, Lee S-T, He Y (2015) Facile, large-quantity synthesis of stable, tunable-color silicon nanoparticles and their application for long-term cellular imaging. ACS Nano 9:5958–5967

    Article  CAS  Google Scholar 

  16. Ma SD, Chen YL, Feng J, Liu JJ, Zuo XW, Chen XG (2016) One-step synthesis of water-dispersible and biocompatible silicon nanoparticles for selective heparin sensing and cell imaging. Anal Chem 88:10474–10481

    Article  CAS  Google Scholar 

  17. Zhang X, Chen X, Kai S, Wang HY, Yang J, Wu FG, Chen Z (2015) Highly sensitive and selective detection of dopamine using one-pot synthesized highly photoluminescent silicon nanoparticles. Anal Chem 87:3360–3365

    Article  CAS  Google Scholar 

  18. Yi Y, Deng J, Zhang Y, Li H, Yao S (2013) Label-free Si quantum dots as photoluminescence probes for glucose detection. Chem Commun 49:612–614

    Article  CAS  Google Scholar 

  19. Yi Y, Zhu G, Liu C, Huang Y, Zhang Y, Li H, Zhao J, Yao S (2013) A label-free silicon quantum dots-based photoluminescence sensor for ultrasensitive detection of pesticides. Anal Chem 85:11464–11470

    Article  CAS  Google Scholar 

  20. Miao L, Zhu C, Jiao L, Li H, Du D, Lin Y, Wei Q (2018) Smart drug delivery system-inspired enzyme-linked immunosorbent assay based on fluorescence resonance energy transfer and allochroic effect induced dual-modal colorimetric and fluorescent detection. Anal Chem 90:1976–1982

    Article  CAS  Google Scholar 

  21. Han Y, Chen Y, Feng J, Liu J, Ma S, Chen X (2017) One-pot synthesis of fluorescent silicon nanoparticles for sensitive and selective determination of 2,4,6-trinitrophenol in aqueous solution. Anal Chem 89:3001–3008

    Article  CAS  Google Scholar 

  22. Lin CY, Yu CJ, Lin YH, Tseng WL (2010) Colorimetric sensing of silver(I) and mercury(II) ions based on an assembly of tween 20-stabilized gold nanoparticles. Anal Chem 82:6830–6837

    Article  CAS  Google Scholar 

  23. Zhu X, Zhao T, Nie Z, Liu Y, Yao S (2015) Non-redox modulated fluorescence strategy for sensitive and selective ascorbic acid detection with highly photoluminescent nitrogen-doped carbon nanoparticles via solid-state synthesis. Anal Chem 87:8524–8530

    Article  CAS  Google Scholar 

  24. Hashemi N, Vaezi Z, Sedghi M, Naderi-Manesh H (2017) Hemoglobin-incorporated iron quantum clusters as a novel fluorometric and colorimetric probe for sensing and cellular imaging of Zn(II) and cysteine. Mikrochim Acta 185:60

    Article  Google Scholar 

  25. Dong Y, Su M, Chen P, Sun H (2015) Chemiluminescence of carbon dots induced by diperiodato-nicklate (IV) in alkaline solution and its application to a quenchometric flow-injection assays of paracetamol, L-cysteine and glutathione. Microchim Acta 182:1071–1077

    Article  CAS  Google Scholar 

  26. Dai H, Shi Y, Wang Y, Sun Y, Hu J, Ni P, Li Z (2014) A carbon dot based biosensor for melamine detection by fluorescence resonance energy transfer. Sensor Actuat B-Chem 202:201–208

    Article  CAS  Google Scholar 

  27. Wang J, Zhang Z, Zha S, Zhu Y, Wu P, Ehrenberg B, Chen JY (2014) Carbon nanodots featuring efficient FRET for two-photon photodynamic cancer therapy with a low fs laser power density. Biomaterials 35:9372–9381

    Article  CAS  Google Scholar 

  28. Zu F, Yan F, Bai Z, Xu J, Wang Y, Huang Y, Zhou X (2017) The quenching of the fluorescence of carbon dots: a review on mechanisms and applications. Microchim Acta 184:1899–1914

    Article  CAS  Google Scholar 

  29. Gu T, Zou W, Gong F, Xia J, Chen C, Chen X (2018) A specific nanoprobe for cysteine based on nitrogen-rich fluorescent quantum dots combined with cu(2). Biosens Bioelectron 100:79–84

    Article  CAS  Google Scholar 

  30. Wu H, Jiang J, Gu X, Tong C (2017) Nitrogen and sulfur co-doped carbon quantum dots for highly selective and sensitive fluorescent detection of Fe(III) ions and L-cysteine. Microchim Acta 184:2291–2298

    Article  CAS  Google Scholar 

  31. Liu M, Li N, He Y, Ge Y, Song G (2018) Dually emitting gold-silver nanoclusters as viable ratiometric fluorescent probes for cysteine and arginine. Mikrochim Acta 185:147

    Article  Google Scholar 

  32. Li Y, Shen C, Li X, Yang M, Shao C (2018) Hydroxyapatite nanoparticle based fluorometric determination and imaging of cysteine and homocysteine in living cells. Mikrochim Acta 185:271

    Article  Google Scholar 

  33. Liu J, Bao C, Zhong X, Zhao C, Zhu L (2010) Highly selective detection of glutathione using a quantum-dot-based OFF-ON fluorescent probe. Chem Commun 46:2971–2973

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We acknowledge the support from the National Natural Science Foundation of China (21607061), the Opening Project of State Key Laboratory of Chemo/Biosensing and Chemometrics of Hunan University (2016017), the Priority Academic Program Development of Jiangsu Higher Education Institutions, Collaborative Innovation Center of Technology and Material of Water Treatment, and the Program of Young Backbone Teachers in Jiangsu University (2015).

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Authors and Affiliations

  1. School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, People’s Republic of China

    Lirong Liu, Gangbing Zhu, Wei Zeng, Yinhui Yi, Baohe Lv, Junjuan Qian & Depeng Zhang

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  1. Lirong Liu
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  2. Gangbing Zhu
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  3. Wei Zeng
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  4. Yinhui Yi
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Correspondence to Yinhui Yi.

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Liu, L., Zhu, G., Zeng, W. et al. Silicon quantum dot-coated onto gold nanoparticles as an optical probe for colorimetric and fluorometric determination of cysteine. Microchim Acta 186, 98 (2019). https://doi.org/10.1007/s00604-019-3228-9

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