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Gold nanoparticle-loaded hollow Prussian Blue nanoparticles with peroxidase-like activity for colorimetric determination of L-lactic acid

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Abstract

The intrinsic peroxidase-like activity of hollow Prussian Blue nanoparticle-loaded with gold nanoparticles (Au@HMPB NPs) were applied to oxidize the substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2 to give a blue-green coloration. The morphology of the Au@HMPB NPs and its peroxidase-mimicking activity was characterized in detail. The catalytic activity follows Michaelis-Menten kinetics and is higher than that of HMPB NPs not loaded with gold nanoparticles. The NPs were employed to detect L-lactic acid colorimetrically (at 450 nm) via detection of H2O2 that is generated during enzymatic oxidation by L-lactate oxidase (LOx). The limit of detection is 4.2 μM. The assay was successfully applied to the quantitation of L-lactic acid in spiker human serum samples.

Gold nanoparticle-loaded hollow Prussian Blue nanoparticles (Au@HMPB NPs) with peroxidase-like catalytic activity can oxidize the substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2. The nanoparticles were applied for the detection of L-lactic acid through detection of H2O2 that is generated by L-lactate oxidase (LOx) catalyzed oxidation of L-lactic acid.

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Acknowledgments

The authors thank the support of this work by the National Natural Science Foundation of China (No. 21575165).

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  1. Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China

    Dandan Zhou, Ke Zeng & Minghui Yang

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  1. Dandan Zhou
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  2. Ke Zeng
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  3. Minghui Yang
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Correspondence to Minghui Yang.

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Zhou, D., Zeng, K. & Yang, M. Gold nanoparticle-loaded hollow Prussian Blue nanoparticles with peroxidase-like activity for colorimetric determination of L-lactic acid. Microchim Acta 186, 121 (2019). https://doi.org/10.1007/s00604-018-3214-7

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