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Teicoplanin-functionalized magnetic beads for detection of Staphylococcus aureus via inhibition of the luminol chemiluminescence by intracellular catalase

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Abstract

An affinity-based protocol is described for the detection of Staphylococcus aureus (S. aureus). It is utilizing teicoplanin-functionalized magnetic beads as carriers. Teicoplanin, which binds to the walls of cells of S. aureus via five hydrogen bonds, acts as the recognition agent. Captured S. aureus is magnetically separated from the sample matrix and then specifically lysed by lysostaphin which cleaves the cross-linking pentaglycine bridges of peptidoglycan in the cell wall. Lastly, S. aureus is quantified via the inhibitory effect of released intracellular catalase on a chemiluminescent (CL) system composed of peroxidase, luminol, H2O2 and p-iodophenol because catalase decomposes H2O2. S. aureus can be detected with CL response in the 140 to 1.4 × 107 CFU·mL−1 concentration range and a detection limit as low as 47 CFU·mL−1 at a signal-to-noise ratio of 3. The method was evaluated by analyzing spiked samples including milk, human urine and saline injection solutions. The reliability was demonstrated by a recovery test and by comparison with a conventional plate counting method.

An antibiotic-affinity protocol is developed to detect Staphylococcus aureus (S. aureus) by utilizing teicoplanin-functionalized magnetic beads (Teic-MBs) as carriers. S. aureus can be quantified by measuring the inhibition of luminol chemiluminescence (CL) signal by intracellular catalase.

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Acknowledgements

This work was financially supported by the Natural Science Foundation of China (21475107) and the Fundamental Research Funds for the Central Universities (XDJK2017A008).

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Authors and Affiliations

  1. Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing, 400716, China

    Yue Wu, Mengyao Wang, Hui Ouyang, Yong He, Xiaoyan Zhao & Zhifeng Fu

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  1. Yue Wu
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  2. Mengyao Wang
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  3. Hui Ouyang
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  4. Yong He
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  5. Xiaoyan Zhao
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Correspondence to Zhifeng Fu.

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Wu, Y., Wang, M., Ouyang, H. et al. Teicoplanin-functionalized magnetic beads for detection of Staphylococcus aureus via inhibition of the luminol chemiluminescence by intracellular catalase. Microchim Acta 185, 391 (2018). https://doi.org/10.1007/s00604-018-2921-4

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