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Synthesis of Au@Ag core-shell nanostructures with a poly(3,4-dihydroxy-L-phenylalanine) interlayer for surface-enhanced Raman scattering imaging of epithelial cells

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Abstract

Poly(3,4-dihydroxy-L-phenylalanine) (polyDOPA) is a stable and biocompatible reducing agent. A versatile strategy is described here for the synthesis of core-shell Au@Ag nanostructures containing a polyDOPA interlayer. The latter provides abundant sites for deposition of nanocomposites, to immobilize molecules and to grow shells. The Au@polyDOPA@Ag nanoparticles are shown to generate strong and stable surface-enhanced Raman spectroscopy (SERS) signals compared to bare AuNPs and bare AgNPs. Folic acid was then immobilized on Au@polyDOPA@Ag nanoparticles and then applied to SERS imaging of human lung adenocarcinoma cell line A549 by the specific recognition of the folic acid receptor. The folic acid-conjugated SERS tags were promising to be nanoplatforms for imaging of cancer cells.

An Au@Ag core-shell nanostructures SERS nanotag with a polyDOPA interlayer was fabricated and then applied to SERS imaging of epithelial cells. (DOPA: 3,4-Dihydroxy-[L-phenylalanine]; FA: folic acid; 4-MBA: 4-mercaptobenzoic acid).

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Acknowledgments

The work was supported by the National Natural Science Foundation of China (Nos. 21575168, 21475153 and 21675178), the Guangdong Provincial Natural Science Foundation of China (Nos. 2015A030311020 and 2017A030313070), and the Special Funds for Public Welfare Research and Capacity Building in Guangdong Province of China (No. 2015A030401036), and the Guangzhou Science and Technology Program of China (Nos. 201704020040 and 201604020165), respectively.

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  1. Haibin Wen and Peichun Jiang contributed equally to this work.

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  1. School of Chemistry, Sun Yat-sen University, Guangzhou, 510275, People’s Republic of China

    Haibin Wen, Peichun Jiang, Yuling Hu & Gongke Li

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  1. Haibin Wen
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Correspondence to Yuling Hu or Gongke Li.

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Wen, H., Jiang, P., Hu, Y. et al. Synthesis of Au@Ag core-shell nanostructures with a poly(3,4-dihydroxy-L-phenylalanine) interlayer for surface-enhanced Raman scattering imaging of epithelial cells. Microchim Acta 185, 353 (2018). https://doi.org/10.1007/s00604-018-2873-8

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