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Voltammetric aptasensor for bisphenol A based on the use of a MWCNT/Fe3O4@gold nanocomposite

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Abstract

The present study describes an electrochemical aptamer-based method for the determination of bisphenol A (BPA). It is making use of gold nanoparticles (AuNPs) immobilized on a conjugate between multiwalled carbon nanotubes and thiol-functionalized magnetic nanoparticles (MWCNT/Fe3O4-SH) that are modified with an aptamer. The nanocomposite was characterized by Fourier transform infrared spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, vibrating sample magnetometry, elemental mapping analysis and energy dispersive X-ray diffraction. The aptasensor, typically operated at 0.20 V (vs. Ag/AgCl), has a linear response in the 0.1 to 8 nM BPA concentration range, a low detection limit (0.03 nM), and high sensitivity (86.43 μA nM−1 cm−2). Voltammetric experiments were performed by using the hexacyanoferrate redox system as an electrochemical probe. The results indicate that the presence of AuNPs, magnetic nanoparticles and MWCNTs results a synergistic electrochemical augmentation. The method is highly selective, sensitive, efficient and environmentally friendly. The method was successfully applied to the determination of BPA in spiked real samples.

Aptasensor fabricated by MWCNT/Fe3O4-SH@Au nanocomposite and anti-BPA aptamer. The conformation of aptamer change after BPA binding, triggering a decrease in the electron transfer of Fe(CN)63−/4− on the electrode surface. The observed decline was detectable as a function of BPA concentration.

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References

  1. Mirzajani H, Cheng C, Wu J, Chen J, Eda S, Aghdam EN, Ghavifekr HB (2017) A highly sensitive and specific capacitive aptasensor for rapid and label-free trace analysis of bisphenol a in canned food. Biosens Bioelectron 89:1059–1067

    Article  CAS  PubMed  Google Scholar 

  2. An analysis of European plastics production, demand and waste data. http://www.plasticseurope.org/Document/plastics-the-facts/, 2014 (accessed 15.01.22)

  3. Hadavifar M, Bahramifar N, Younesi H, Li Q (2014) Adsorption of mercury ions from synthetic and real wastewater aqueous solution by functionalized multi-walled carbon nanotube with both amino and thiolated groups. Biochem Eng J 237:217

    CAS  Google Scholar 

  4. Temel NK, Gürkan R (2017) A micellar sensitized kinetic method for quantification of low levels of bisphenol a in foodstuffs by spectrophotometry. Anal Methods 9:1190–1200

    Article  CAS  Google Scholar 

  5. Filippou O, Deliyanni EA, Samanidou VF (2016) Fabrication and evaluation of magnetic activated carbon as adsorbent for ultrasonic assisted magnetic solid phase dispersive extraction of bisphenol a form milk prior to high performance liquid chromatographic analysis with ultraviolet detection. J Chromatogr A 1479:20

    Article  CAS  PubMed  Google Scholar 

  6. Zimmers SM, Browne EP, O’Keefe PW, Anderton DL, Kramer L, Reckhow DA, Arcaro KF (2014) Determination of free bisphenol a concentrations in breast milk of U.S. women using a sensitive LC/MS/MS method. Chemosphere 104:237

    Article  CAS  PubMed  Google Scholar 

  7. Pastor-Belda M, Bastida D, Campillo N, Pérez-Cárceles MD, Motas M, Vinas P (2016) A study of the influence on diabetes of free and conjugated bisphenol a concentration in urine: development of a simple microextraction procedure using gas chromatography–mass spectrometry. J Pharm Biomed Anal 129:458–465

    Article  CAS  PubMed  Google Scholar 

  8. Zhang X, Zhu D, Huang C, Sun Y, Lee Y (2015) Sensitive detection of bisphenol a in complex samples by in-column molecularly imprinted solid-phase extraction coupled with capillary electrophoresis. Microchem J 121:1–5

    Article  CAS  Google Scholar 

  9. Xue F, Wu J, Chu H, Mei Z, Ye Y, Liu J, Zhang R, Peng C, Chen W (2013) Electrochemical aptasensor for the determination of bisphenol a in drinking water. Microchim Acta 180:109–115

    Article  CAS  Google Scholar 

  10. Li O, Zhai X, Liu X, Wang L, Liu H, Wang H (2016) Electrochemical determination of bisphenol a at ordered mesoporous carbon modified nano-carbon ionic liquid paste electrode. Talanta 148:362–369

    Article  CAS  PubMed  Google Scholar 

  11. Alsaafin A, McKeague M (2017) Functional nucleic acids as in vivo metabolite and ion biosensors. Biosens Bioelectron 94:94–106

    Article  CAS  PubMed  Google Scholar 

  12. Rapini R, Marrazza G (2017) Electrochemical aptasensors for contaminants detection in food and environment: recent advances. Bioelectrochemistry 118:47–61

    Article  CAS  PubMed  Google Scholar 

  13. Zhu Y, Zhou C, Yan X, Yan Y, Wang Q (2015) Aptamer-functionalized nanoporous gold film for high-performance direct electrochemical detection of bisphenol a in human serum. Anal Chim Acta 883:81–89

    Article  CAS  PubMed  Google Scholar 

  14. Liu Y, Zhang X, Yang J, Xiong E, Zhang X, Chen J (2016) Sensitive detection of bisphenol a based on a ratiometric electrochemical aptasensor. Can J Chem 94:509–514

    Article  CAS  Google Scholar 

  15. Derikvand Z, Abbasi AR, Roushani M, Derikvand Z, Azadbakht Z (2016) Design of ultra- sensitive bisphenol A-aptamer based on Pt nanoparticles loading to polyethyleneimine functionalized carbon nanotubes. Anal Biochem 512:47–57

    Article  CAS  PubMed  Google Scholar 

  16. Cui H, Wu J, Eda S, Chen J, Chen W, Zheng L (2015) Rapid capacitive detection of femtomolar levels of bisphenol a using an aptamer-modified disposable microelectrode array. Microchim Acta 182:2361–2367

    Article  CAS  Google Scholar 

  17. Ebrahimpour E, Amiri A, Baghayeri M, Rouhi M, Lakouraj MM (2017) Poly (indole-co-thiophene)@Fe3O4 as novel adsorbents for the extraction of aniline derivatives from water samples. Microchem J 131:174–181

    Article  CAS  Google Scholar 

  18. Rouhi M, Lakouraj MM, Baghayeri M, Hasantabar V (2017) Novel conductive magnetic nanocomposite based on poly (indole-co-thiophene) as a hemoglobin diagnostic biosensor: synthesis, characterization and physical properties. Int J Polymer Mater 66:12–19

    Article  CAS  Google Scholar 

  19. Baghayeri M, Zare EN, Lakouraj MM (2014) A simple hydrogen peroxide biosensor based on a novel electro-magnetic poly(p-phenylenediamine)@Fe3O4 nanocomposite. Biosens Bioelectron 55:259–265

    Article  CAS  PubMed  Google Scholar 

  20. Wang J (2012) Electrochemical biosensing based on noble metal nanoparticles. Microchim Acta 177:245–270

    Article  CAS  Google Scholar 

  21. Tian Y, Chen L, Zhang J, Ma Z, Song C (2012) Bifunctional au-nanorod@Fe3O4 nanocomposites: synthesis, characterization, and their use as bioprobes. J Nanopart Res 14:998

    Article  CAS  Google Scholar 

  22. Zhou L, Wang J, Li D, Li Y (2014) An electrochemical aptasensor based on gold nanoparticles dotted graphene modified glassy carbon electrode for label-free detection of bisphenol a in milk samples. Food Chem 162:34–40

    Article  CAS  PubMed  Google Scholar 

  23. Deiminiat B, Rounaghi GH, Arbab-Zavar MH, Razavipanah I (2016) A novel electrochemical aptasensor based on f-MWCNTs/AuNPs nanocomposite for label-free detection of bisphenol a. Sensors Actuators B Chem 242:158

    Article  CAS  Google Scholar 

  24. Thakur H, Kaur N, Sareen D, Prabhakar N (2017) Electrochemical determination of M. Tuberculosis antigen based on poly(3,4-ethylenedioxythiophene) and functionalized carbon nanotubes hybrid platform. Talanta 171:115

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We would like to thank the post-graduate office of Guilan University and Hakim Sabzevari University for the support of this work.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Hakim Sabzevari University, PO. Box 397, Sabzevar, Iran

    Mehdi Baghayeri

  2. Department of Chemistry, Faculty of Science, University of Guilan, Namjoo Street, PO. Box 1914, Rasht, Iran

    Reza Ansari & Marzieh Nodehi

  3. Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

    Iman Razavipanah

  4. Department of Chemistry, Payame Noor University, Tehran, 19395-4697, Iran

    Hojat Veisi

Authors
  1. Mehdi Baghayeri
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  2. Reza Ansari
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  3. Marzieh Nodehi
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  4. Iman Razavipanah
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  5. Hojat Veisi
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Correspondence to Mehdi Baghayeri.

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Baghayeri, M., Ansari, R., Nodehi, M. et al. Voltammetric aptasensor for bisphenol A based on the use of a MWCNT/Fe3O4@gold nanocomposite. Microchim Acta 185, 320 (2018). https://doi.org/10.1007/s00604-018-2838-y

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  • DOI: https://doi.org/10.1007/s00604-018-2838-y

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