Microchimica Acta

, 185:241 | Cite as

Nanosized aptameric cavities imprinted on the surface of magnetic nanoparticles for high-throughput protein recognition

  • Elham Shoghi
  • Seyede Zohreh Mirahmadi-Zare
  • Razieh Ghasemi
  • Matine Asghari
  • Mansour Poorebrahim
  • Mohammad-Hossein Nasr-Esfahani
Original Paper

Abstract

The authors introduce a new kind of surface artificial biomimetic receptor, referred to as aptameric imprinted polymer (AIP), for separation of biological macromolecules. Highly dispersed magnetic nanoparticles (MNPs) were coated with silica and then functionalized with methacrylate groups via silane chemistry. The aptamer was covalently immobilized on the surface of nanoparticles via a “thiol-ene” click reaction. Once the target analyte (bovine serum albumin; BSA) has bound to the aptamer, a polymer is created by 2-dimensional copolymerization of short-length poly(ethylene glycol) and (3-aminopropyl)triethoxysilane. Following removal of BSA from the polymer, the AIP-MNPs presented here can selectively capture BSA with a specific absorbance (κ) as high as 65. When using this AIP, the recovery of BSA from spiked real biological samples is >97%, and the adsorption capacity is as high as 146 mg g−1. In our perception, this method has a wide scope in that it may be applied to the specific extraction of numerous other biomolecules.

Graphical abstract

Schematic presentation of the AIP (aptamer-imprinted polymer) introduced here. The surface of silica coated magnetic nanoparticles is modified with a polymer that is covalently modified with an aptamer against bovine serum albumin (BSA).

Keywords

Affinity separation Protein-aptamer sensing Solid phase extraction Size exclusion Bovine serum albumin 

Notes

Acknowledgments

The authors gratefully acknowledge the financial support from the National Elite’s Foundation (BMN) and Iran National Science Foundation (INSF).

Compliance with ethical standards

The author(s) declare that they have no competing interests.

Supplementary material

604_2018_2745_MOESM1_ESM.docx (368 kb)
ESM 1 (DOCX 367 kb)

References

  1. 1.
    Rezaei B, Mirahmadi-Zare S (2011) Nanoscale manipulation of prednisolone as electroactive configuration using molecularly imprinted-multiwalled carbon nanotube paste electrode. Electroanalysis 23(11):2724–2734CrossRefGoogle Scholar
  2. 2.
    Whitcombe MJ, Chianella I, Larcombe L, Piletsky SA, Noble J, Porter R, Horgan A (2011) The rational development of molecularly imprinted polymer-based sensors for protein detection. Chem Soc Rev 40(3):1547–1571CrossRefGoogle Scholar
  3. 3.
    Bossi A, Bonini F, Turner A, Piletsky S (2007) Molecularly imprinted polymers for the recognition of proteins: the state of the art. Biosens Bioelectron 22(6):1131–1137CrossRefGoogle Scholar
  4. 4.
    Zhou X, Li W, He X, Chen L, Zhang Y (2007) Recent advances in the study of protein imprinting. Sep Purif Rev 36(4):257–283CrossRefGoogle Scholar
  5. 5.
    Gao R, Kong X, Wang X, He X, Chen L, Zhang Y (2011) Preparation and characterization of uniformly sized molecularly imprinted polymers functionalized with core–shell magnetic nanoparticles for the recognition and enrichment of protein. J Mater Chem 21(44):17863–17871CrossRefGoogle Scholar
  6. 6.
    Gai Q-Q, Qu F, Zhang T, Zhang Y-K (2011) The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation. J Chromatogr A 1218(22):3489–3495CrossRefGoogle Scholar
  7. 7.
    Lin Z, Xia Z, Zheng J, Zheng D, Zhang L, Yang H, Chen G (2012) Synthesis of uniformly sized molecularly imprinted polymer-coated silica nanoparticles for selective recognition and enrichment of lysozyme. J Mater Chem 22(34):17914–17922CrossRefGoogle Scholar
  8. 8.
    Li L, He X, Chen L, Zhang Y (2009) Preparation of core-shell magnetic molecularly imprinted polymer nanoparticles for recognition of bovine hemoglobin. Chem Asian J 4(2):286–293CrossRefGoogle Scholar
  9. 9.
    Zhou W-H, Lu C-H, Guo X-C, Chen F-R, Yang H-H, Wang X-R (2010) Mussel-inspired molecularly imprinted polymer coating superparamagnetic nanoparticles for protein recognition. J Mater Chem 20(5):880–883CrossRefGoogle Scholar
  10. 10.
    Lu C-H, Zhang Y, Tang S-F, Fang Z-B, Yang H-H, Chen X, Chen G-N (2012) Sensing HIV related protein using epitope imprinted hydrophilic polymer coated quartz crystal microbalance. Biosens Bioelectron 31(1):439–444CrossRefGoogle Scholar
  11. 11.
    Nishino H, Huang CS, Shea KJ (2006) Selective protein capture by epitope imprinting. Angew Chem Int Ed 45(15):2392–2396CrossRefGoogle Scholar
  12. 12.
    Tai D-F, Lin C-Y, Wu T-Z, Chen L-K (2005) Recognition of dengue virus protein using epitope-mediated molecularly imprinted film. Anal Chem 77(16):5140–5143CrossRefGoogle Scholar
  13. 13.
    Yang Y-Q, He X-W, Wang Y-Z, Li W-Y, Zhang Y-K (2014) Epitope imprinted polymer coating CdTe quantum dots for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin. Biosens Bioelectron 54:266–272CrossRefGoogle Scholar
  14. 14.
    Kempe M, Glad M, Mosbach K (1995) An approach towards surface imprinting using the enzyme ribonuclease a. J Mol Recognit 8(1–2):35–39CrossRefGoogle Scholar
  15. 15.
    Poma A, Guerreiro A, Whitcombe MJ, Piletska EV, Turner AP, Piletsky SA (2013) Solid-phase synthesis of molecularly imprinted polymer nanoparticles with a reusable template–“plastic antibodies”. Adv Funct Mater 23(22):2821–2827CrossRefGoogle Scholar
  16. 16.
    Poma A, Guerreiro A, Caygill S, Moczko E, Piletsky S (2014) Automatic reactor for solid-phase synthesis of molecularly imprinted polymeric nanoparticles (MIP NPs) in water. RSC Adv 4(8):4203–4206CrossRefGoogle Scholar
  17. 17.
    Kan X, Zhao Q, Shao D, Geng Z, Wang Z, Zhu J-J (2010) Preparation and recognition properties of bovine hemoglobin magnetic molecularly imprinted polymers. J Phys Chem B 114(11):3999–4004CrossRefGoogle Scholar
  18. 18.
    Gai Q-Q, Qu F, Liu Z-J, Dai R-J, Zhang Y-K (2010) Superparamagnetic lysozyme surface-imprinted polymer prepared by atom transfer radical polymerization and its application for protein separation. J Chromatogr A 1217(31):5035–5042CrossRefGoogle Scholar
  19. 19.
    Mirahmadi-Zare SZ, Allafchian A, Aboutalebi F, Shojaei P, Khazaie Y, Dormiani K, Lachinani L, Nasr-Esfahani M-H (2016) Super magnetic nanoparticles NiFe2O4, coated with aluminum–nickel oxide sol-gel lattices to safe, sensitive and selective purification of his-tagged proteins. Protein Expr Purif 121:52–60CrossRefGoogle Scholar
  20. 20.
    Poma A, Brahmbhatt H, Pendergraff HM, Watts JK, Turner NW (2015) Generation of novel hybrid aptamer–molecularly imprinted polymeric nanoparticles. Adv Mater 27(4):750–758CrossRefGoogle Scholar
  21. 21.
    Liu X, Ren J, Su L, Gao X, Tang Y, Ma T, Zhu L, Li J (2017) Novel hybrid probe based on double recognition of aptamer-molecularly imprinted polymer grafted on upconversion nanoparticles for enrofloxacin sensing. Biosens Bioelectron 87:203–208CrossRefGoogle Scholar
  22. 22.
    Brahmbhatt H, Poma A, Pendergraff H, Watts J, Turner N (2016) Improvement of DNA recognition through molecular imprinting: hybrid oligomer imprinted polymeric nanoparticles (oligoMIP NPs). Biomater Sci 4(2):281–287CrossRefGoogle Scholar
  23. 23.
    Wang Z, J-c Z, H-z L, H-y C (2015) Aptamer-based organic-silica hybrid affinity monolith prepared via “thiol-ene” click reaction for extraction of thrombin. Talanta 138:52–58CrossRefGoogle Scholar
  24. 24.
    Hui C, Shen C, Yang T, Bao L, Tian J, Ding H, Li C, Gao H-J (2008) Large-scale Fe 3O 4 nanoparticles soluble in water synthesized by a facile method. J Phys Chem C 112(30):11336–11339CrossRefGoogle Scholar
  25. 25.
    Stöber W, Fink A, Bohn E (1968) Controlled growth of monodisperse silica spheres in the micron size range. J Colloid Interface Sci 26(1):62–69CrossRefGoogle Scholar
  26. 26.
    Kryscio DR, Peppas NA (2012) Surface imprinted thin polymer film systems with selective recognition for bovine serum albumin. Anal Chim Acta 718:109–115CrossRefGoogle Scholar
  27. 27.
    Kim K-M, Kim HM, Lee W-J, Lee C-W, T-i K, Lee J-K, Jeong J, Paek S-M, Oh J-M (2014) Surface treatment of silica nanoparticles for stable and charge-controlled colloidal silica. Int J Nanomedicine 9(Suppl 2):29Google Scholar
  28. 28.
    Ruff P, Pai RB, Storici F (2012) Real-time PCR-coupled CE-SELEX for DNA aptamer selection. ISRN Mol Biol 2012:1–9CrossRefGoogle Scholar
  29. 29.
    Ye L, Cormack PA, Mosbach K (1999) Molecularly imprinted monodisperse microspheres for competitive radioassay. Anal Commun 36(2):35–38CrossRefGoogle Scholar
  30. 30.
    Li X, Zhang B, Li W, Lei X, Fan X, Tian L, Zhang H, Zhang Q (2014) Preparation and characterization of bovine serum albumin surface-imprinted thermosensitive magnetic polymer microsphere and its application for protein recognition. Biosens Bioelectron 51:261–267CrossRefGoogle Scholar
  31. 31.
    Xue Z, Xi-Wen H, Lang-Xing C, Wen-You L, ZHANG Y-K (2009) Optimum conditions of separation selectivity based on molecularly imprinted polymers of bovine serum albumin formed on surface of Aminosilica. Chin J Anal Chem 37(2):174–180CrossRefGoogle Scholar
  32. 32.
    Ran D, Wang Y, Jia X, Nie C (2012) Bovine serum albumin recognition via thermosensitive molecular imprinted macroporous hydrogels prepared at two different temperatures. Anal Chim Acta 723:45–53CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Molecular Biotechnology, Cell Science Research CenterRoyan Institute for Biotechnology, ACECRIsfahanIran

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