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Preparation of surface imprinted core-shell particles via a metal chelating strategy: specific recognition of porcine serum albumin

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Abstract

We describe the synthesis of molecularly imprinted core-shell microparticles via a metal chelating strategy that assists in the creation of selective recognition sites for albumin. Porcine serum albumin (PSA) was immobilized on silica beads via copper(II) chelation interaction. A solution containing 2-hydroxyethyl methacrylate and methacrylic acid as the monomers was mixed with the above particles, and free radical polymerization was performed at 25 °C. Copper ion and template were then removed to obtain PSA-imprinted core-shell particles (MIPs) with a typical diameter of 5 μm. The binding capacity of such MIP was 8.9 mg protein per gram of MIPs, and the adsorption equilibrium was established within <20 min. The imprinting factor for PSA reached 2.6 when the binding capacity was 7.7 mg protein per gram of MIPs. The use of such MIPs enabled PSA to be selectively recognized even in presence of the competitive proteins ribonuclease B, cytochrome c, and myoglobin. The results indicate that this imprinting strategy for protein may become a promising method to prepare MIPs for protein recognition.

Preparation and rebinding of surface imprinted particles for target protein recognition

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Acknowledgments

This work was supported by the National Basic Research Program of China (2012CB910601 and 2013CB911202), National Nature Science Foundation (21375128 and 21190043), the Creative Research Group Project of the NSFC (21321064), and the National High Technology Research and Development Program of China (2012AA020202).

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Authors and Affiliations

  1. Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R.&.A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China

    Qinran Li, Kaiguang Yang, Senwu Li, Lukuan Liu, Lihua Zhang, Zhen Liang & Yukui Zhang

  2. University of Chinese Academy of Sciences, Beijing, 100039, China

    Qinran Li, Senwu Li & Lukuan Liu

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  1. Qinran Li
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  2. Kaiguang Yang
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  3. Senwu Li
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  4. Lukuan Liu
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  5. Lihua Zhang
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  6. Zhen Liang
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  7. Yukui Zhang
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Correspondence to Lihua Zhang.

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Li, Q., Yang, K., Li, S. et al. Preparation of surface imprinted core-shell particles via a metal chelating strategy: specific recognition of porcine serum albumin. Microchim Acta 183, 345–352 (2016). https://doi.org/10.1007/s00604-015-1640-3

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  • DOI: https://doi.org/10.1007/s00604-015-1640-3

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