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Chitosan stabilized gold nanoparticle based electrochemical ractopamine immunoassay

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Abstract

The authors describe an electrochemical immunoassay for the β-adrenergic agonist ractopamine (RAC). It is based on the use of chitosan-stabilized gold nanoparticles (chit-AuNPs). Antibodies against RAC (anti-RAC) readily adsorb on the surface of the chit-AuNPs due to their high bio-affinity. The amount of chitosan used to synthesize chit-AuNPs does not affect the size and distribution of the NPs but amplifies the electrochemical signal of the electrode. The assay has a detection limit as low as 2.3 pg∙mL−1 (equivalent to a 6.7 fM concentration), with a response that is linear in the 0.01 to 5 ng∙mL−1 RAC concentration range. The assay is selective, acceptably reproducible, stable, and well applicable to the detection of RAC.

Chitosan-stabilized gold nanoparticles (chit-AuNPs) were prepared for electrochemical immunoassay of the ractopamine (RAC). The assay exhibits a detection limit of 2.3 pg·mL−1 (equal to 6.7 fM), is highly selective, acceptably reproducible, and stable.

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Acknowledgements

This work was supported by Programs for the National Natural Science Foundation of China (NSFC: Account No. U1604127 and 21601161) and Innovative Technology Team of Henan Province (CXTD2014042).

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Authors and Affiliations

  1. Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou, 450001, China

    Linghao He, Chuanpan Guo, Yingpan Song, Shuai Zhang, Minghua Wang, Donglai Peng, Shaoming Fang, Zhihong Zhang & Chun-Sen Liu

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  1. Linghao He
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  2. Chuanpan Guo
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  3. Yingpan Song
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  4. Shuai Zhang
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  5. Minghua Wang
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  6. Donglai Peng
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  7. Shaoming Fang
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  8. Zhihong Zhang
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  9. Chun-Sen Liu
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Correspondence to Zhihong Zhang or Chun-Sen Liu.

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He, L., Guo, C., Song, Y. et al. Chitosan stabilized gold nanoparticle based electrochemical ractopamine immunoassay. Microchim Acta 184, 2919–2924 (2017). https://doi.org/10.1007/s00604-017-2315-z

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  • DOI: https://doi.org/10.1007/s00604-017-2315-z

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