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SiO2-MIP core-shell nanoparticles containing gold nanoclusters for sensitive fluorescence detection of the antibiotic erythromycin

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Abstract

The authors describe a fluorescence based assay for determination of the macrolid antibiotic erythromycin (ERY). It is based in the use of fluorescent gold nanoclusters (AuNCs) coated first with silica (to obtain AuNC@SiO2) and then with a molecularly imprinted polymer (MIP). The MIP was synthesized from 3-aminopropyltriethoxysilane as the functional monomer, ERY as the template, and tetraethoxysilane as the cross-linker via a sol-gel process that leads to surface imprinting. By using this method, the strong fluorescence of AuNCs was maintained in the resultant MIPs. After optimization of the experimental conditions, the signal (measured at excitation/emission wavelengths of 396/585 nm) decreases linearly with increasing concentration of ERY in the 0.1 to 11.9 μM ERY concentration range. The limit of detection is 12 nM, and the imprinting factor is 4.0. The method was successfuly applied to the determination of ERY in spiked human urine and saliva.

Schematic of the preparation of a core-shell sensor material with the silica coated by fluorescent gold nanoclusters as core and a molecularly imprinted polymer. It can be used to selectively detect residual erythromycin in biological samples.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (Nos. U1507118, U1407123, 21406085) and Natural Science Foundation of Jiangsu Province (Nos. BK20151350, BK20140580, BK20161367), China Postdoctoral Science Foundation Funded Project (Nos. 2014 M561588, 1501067C).

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Authors and Affiliations

  1. School of Material Science and Engineering, Jiangsu University, Zhenjiang, 212013, China

    Yufeng Zhang, Zhiping Zhou, Jiuyun Cui & Siwei Liu

  2. School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, China

    Jiahong Zheng, Hongji Li, Yongsheng Yan & Chunxiang Li

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  1. Yufeng Zhang
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  2. Zhiping Zhou
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Correspondence to Zhiping Zhou.

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Zhang, Y., Zhou, Z., Zheng, J. et al. SiO2-MIP core-shell nanoparticles containing gold nanoclusters for sensitive fluorescence detection of the antibiotic erythromycin. Microchim Acta 184, 2241–2248 (2017). https://doi.org/10.1007/s00604-017-2216-1

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