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Determination of the activity of superoxide dismutase using a glassy carbon electrode modified with ferrocene imidazolium salts and hydroxy-functionalized graphene

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Abstract

A glassy carbon electrode was modified with a composite consisting of ferrocene imidazolium salts and hydroxy-functionalized graphene in a Nafion matrix. The electrode is shown to facilitate the reduction of oxygen to form the superoxide anion radical. Atomic force microscopy and electrochemical impedance spectroscopy were used to characterize the electrode. The in-situ production of superoxide anion was then studied with an analogue of methyl-Cypridina luciferin (also referred to as Vargulin). As a result, an electrochemiluminescence (ECL) based assay was developed for the improved determination of the activity of the enzyme superoxide dismutase (SOD). Under optimized conditions, the ECL based calibration plot is linear in the 0.5 to 6.5 U·mL−1 SOD activity range, with a 0.2 U·mL−1 detection limit (at a signal-to-noise ratio of 3).

Schematic of the detection scheme. The surface of a glassy carbon electrode is modified with a modified ferrocenyl-methyl-imidazolium ionic liquid and hydroxy-functionalized graphene. The release of superoxide radical is detected via the electrochemiluminescence of MCLA (a methyl cypridina luciferin analogue)

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Acknowledgments

This project was financially supported by the Scientific and Technological Foundation of Fujian Province of China (2011 J01043), Education Ministry of Fujian Province of China (JA15057) and Fuzhou University (2014-XQ-8).

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Authors and Affiliations

  1. Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian, 350108, China

    Jian Wang & Minyan Liu

  2. College of Chemistry, Fuzhou University, Qi Shan Campus, Fuzhou, Fujian, 350108, China

    Jian Wang, Zhou Jiang, Lili Xie, Minyan Liu & Yaofeng Yuan

Authors
  1. Jian Wang
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  2. Zhou Jiang
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  3. Lili Xie
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  4. Minyan Liu
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Correspondence to Jian Wang or Yaofeng Yuan.

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Wang, J., Jiang, Z., Xie, L. et al. Determination of the activity of superoxide dismutase using a glassy carbon electrode modified with ferrocene imidazolium salts and hydroxy-functionalized graphene. Microchim Acta 184, 289–296 (2017). https://doi.org/10.1007/s00604-016-2018-x

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