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Differential pulse voltammetric assay for the carcinoembryonic antigen using a glassy carbon electrode modified with layered molybdenum selenide, graphene, and gold nanoparticles

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Abstract

A highly sensitive electrochemical aptasensor is described for the determination of carcinoembryonic antigen (CEA). It is based on the use of a hybrid material composed of molybdenum selenide, graphene, and gold nanoparticles (AuNPs). The MoSe2-graphene hybrid was prepared by a hydrothermal method and used as the supporting substrate. It was placed on the surface of a glassy carbon electrode onto which the AuNPs were electrochemically deposited. Thiol-labeled aptamer against CEA was immobilized on the electrode via gold-thiol binding. The use of AuNPs coupled to the MoSe2-graphene hybrid allows for a large loading with aptamers. Under optimum conditions and at a working potential of 0.21 V (vs. SCE), the assay has a linear calibration plot in the 0.1 pg mL−1 to 100 ng mL−1 CEA concentration range, with a detection limit of 0.03 pg mL−1.

Schematic of an electrochemical aptasensor for determination of carcinoembryonic antigen using a glassy carbon electrode modified with layered molybdenum selenide, graphene, and gold nanoparticles.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (Grant No. 61301037), Youth Backbone Teacher Training Program of Henan University of Technology.

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  1. School of Food Science and Technology, Henan University of Technology, Zhengzhou, 450001, China

    Baoshan He

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  1. Baoshan He
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Correspondence to Baoshan He.

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He, B. Differential pulse voltammetric assay for the carcinoembryonic antigen using a glassy carbon electrode modified with layered molybdenum selenide, graphene, and gold nanoparticles. Microchim Acta 184, 229–235 (2017). https://doi.org/10.1007/s00604-016-2006-1

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  • DOI: https://doi.org/10.1007/s00604-016-2006-1

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