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An aptamer based assay for the β-adrenergic agonist ractopamine based on aggregation of gold nanoparticles in combination with a molecularly imprinted polymer

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Abstract

A sensitive visual aptamer-based assay is presented for the determination of ractopamine (RAC) in animal feed beef. In the absence of RAC, the aptamer binds to gold nanoparticles (AuNPs) and this prevents the AuNPs to undergo salt-induced aggregation which usually is accompanied by a color change from red to blue. If however, RAC is present, it will bind to the aptamer while the AuNPs remain uncoated so that aggregation and a color change will occur due to salt-induced aggregation. This can be monitored by spectrophotometer or even with bare eyes. Under optimal conditions, the aptasensor exhibits a linear range that covers the 10 to 400 ng.mL‾1 RAC concentration range. The limit of detection is as low as 10 ng.mL‾1. In order to further improve selectivity, a RAC-selective molecularly imprinted membrane was prepared and used to pre-extract RAC from complex samples. The combined method (molecularly imprinted membrane and aptasensor) was applied to the determination of RAC in spiked animal feed and beef and gave recoveries that ranged from 72.7 % to 87.3 % for complete feed and from 78.2 % to 86.5 % for beef, respectively.

A sensitive visual aptamer-based assay based on aggregation of gold nanoparticles in combination with a molecularly imprinted polymer was developed for the determination of ractopamine (RAC) in animal feed and beef.

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Acknowledgments

The authors would like to thank the Special Fund for Agro-scientific Research in the Public Interest (No. 201203088), the NSFC (No.31201832), the National S&T pillar project (No.2011BAD26B0405), the International Cooperation Program (2012DFA31140), and Beijing Special Program for Food Safety (No. Z151100001215002) for financially supporting of this research.

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Authors and Affiliations

  1. Institute of Quality Standards and Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Peilong Wang & Xiaoou Su

  2. Department of Chemistry, Liaoning Normal University, Dalian, 116029, China

    Lei Shi

  3. Institute of Quality and Standards for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Yuwei Yuan

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  1. Peilong Wang
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  2. Xiaoou Su
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  4. Yuwei Yuan
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Correspondence to Xiaoou Su or Yuwei Yuan.

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Wang, P., Su, X., Shi, L. et al. An aptamer based assay for the β-adrenergic agonist ractopamine based on aggregation of gold nanoparticles in combination with a molecularly imprinted polymer. Microchim Acta 183, 2899–2905 (2016). https://doi.org/10.1007/s00604-016-1913-5

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  • DOI: https://doi.org/10.1007/s00604-016-1913-5

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