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SERS-based rapid detection of 2,4-dichlorophenoxyacetic acid in food matrices using molecularly imprinted magnetic polymers

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Abstract

In order to remove the limitations of natural antibodies or enzymes, a nano-magnetic biomimetic platform based on a surface-enhanced Raman scattering (SERS) sensor has been developed for highly sensitive capture and detection of 2,4-dichlorophenoxyacetic acid (2,4-D) in food and water samples. Magnetic-based molecular imprinted polymer nanoparticles (Mag@MIP NPs) were constructed to capture the target 2,4-D molecule via biomimetic recognition, and gold nanoparticles (Au NPs) served as SERS-based probes, which are bound to the Mag@MIP NPs by electrostatic adsorption. The as-prepared SERS-MIP sensor for sensing of 2,4-D achieved a good linear relationship with a low detection limit (LOD) of 0.00147 ng/mL within 2 h and exhibited high sensitivity. The sensor was successfully applied to detect 2,4-D in milk and tap water and achieved good recoveries ranging from 93.5 to 102.2%. Moreover, the designed sensor system exhibited satisfactory results (p > 0.05) compared to HPLC by validation analysis. Hence, the findings demonstrated that the proposed method has significant potential for practical application in food safety and environmental protection.

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Funding

This work has been financially supported by the National Natural Science Foundation of China (31772063, 31901772), and the Key R&D Program of Jiangsu Province (BE2017302).

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  1. Yi Xu and Md Mehedi Hassan These authors contributed equally to this work and should be considered co-first authors.

Authors and Affiliations

  1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, People’s Republic of China

    Yi Xu, Md Mehedi Hassan, Shujat Ali, Huanhuan Li & Quansheng Chen

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  1. Yi Xu
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  2. Md Mehedi Hassan
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  3. Shujat Ali
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  4. Huanhuan Li
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Correspondence to Huanhuan Li or Quansheng Chen.

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Xu, Y., Hassan, M.M., Ali, S. et al. SERS-based rapid detection of 2,4-dichlorophenoxyacetic acid in food matrices using molecularly imprinted magnetic polymers. Microchim Acta 187, 454 (2020). https://doi.org/10.1007/s00604-020-04408-2

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