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Separation and stacking of iodine species from seafood using surfactant-coated multiwalled carbon nanotubes as a pseudo-stationary phase in capillary electrophoresis

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Abstract

This article describes an on-line technique for hydrodynamic injections of long sample plugs with simultaneous stacking of iodine species in capillary electrophoresis (CE) with diode array detection. Surfactant-coated multi-walled carbon nanotubes (SC-MWNTs) were used as a pseudostationary phase for the separation of iodate, tetraiodothyronine,triiodothyronine, diiodothyronine, and diiodotyrosine. The effects of MWNTs, concentration of SC-MWNTs and salt, of buffer pH value, injection times were examined. Under the optimized conditions, i.e. detection at 230 nm, a separation voltage 25 kV, a borate running buffer of pH 7.5 and a SC-MWNT concentration of 9 μg · mL−1, the method gave relative standard deviations of the retention times and peak areas in intra-day assays (for n = 6) and for inter-day assays (for n = 3) of less than 4.49 and 5.80 %, respectively. The CE method was then applied to the analysis of the above iodine species in (spiked) kelp (kunbu) and porphyra, and recoveries ranged from 81.6 to 98.4 % with RSDs (n = 3) for extraction repeatability of <3.39 % in all cases.

Surfactant coated multi-walled carbon nanotubes were used as a pseudostationary phase to improve separation in iodine speciation. A highly sensitive stacking method was developed to enhance the detection sensitivity of iodine species. The CE method was then applied to the analysis of iodine species in kelp and porphyra.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 81274065), the Zhejiang Provincial Natural Science Foundation of China (LY15H280016), the Hangzhou social development of scientific research projects (No. 20150533B05), Research on Public Welfare Technology Application Projects of Zhejiang Province (No. 2014C37069), Young and Middle-Aged Academic Leaders of Hangzhou (2013–45), and Changjiang Scholars and Innovative Research Team in Chinese University (IRT 1231).

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Authors and Affiliations

  1. College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 310036, China

    Jing-Jing Xu, Jun Cao & Li-Qing Peng

  2. Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China

    Yan-xu Chang

  3. Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI, 48109, USA

    Jie Hao

  4. Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI, 48109, USA

    Mingrui An, Zhijing Tan & Rui Yang

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  1. Jing-Jing Xu
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Correspondence to Yan-xu Chang or Jun Cao.

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Xu, JJ., Chang, Yx., Hao, J. et al. Separation and stacking of iodine species from seafood using surfactant-coated multiwalled carbon nanotubes as a pseudo-stationary phase in capillary electrophoresis. Microchim Acta 183, 2441–2447 (2016). https://doi.org/10.1007/s00604-016-1892-6

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