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MoS2 based digital response platform for aptamer based fluorescent detection of pathogens

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Abstract

The authors describe a method for detecting the pathogen S. typhimurium with a ‘yes-or-no’ kind of response. A fluorescein-tagged aptamer (Apt-FAM) probe was used for the specific recognition of pathogens, and molybdenum(IV) disulfide nanosheets (MoS2-Ns) were used as a primary binder of free (unbound) ssDNA that quenches its fluorescence. If, however, target pathogens are present, Apt-FAM probe binds to it and fluorescence is restored. The method displays a detection limit of 10 CFU·mL−1, is selective over E. coli and P. vulgaris, and therefore represents a promising on-site screening tool for microbes.

Molybdenum disulfide nanosheets (MoS2-Ns) act as primary binder of free fluorophore labelled ssDNA aptamer and quenches its fluorescence. If target pathogens are present aptamer binds to it instead of MoS2-Ns and fluorescence is restored.

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Acknowledgments

The authors thank Director, IMTECH for generous startup funds. VB thanks DST for YS grant.

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Authors and Affiliations

  1. CSIR-Institute of Microbial Technology, Chandigarh, 160036, India

    Pargat Singh, Ritika Gupta, Monika Sinha & Vijayender Bhalla

  2. UIET-Panjab University, Chandigarh, 160014, India

    Pargat Singh & Rajesh Kumar

Authors
  1. Pargat Singh
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  2. Ritika Gupta
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  3. Monika Sinha
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  4. Rajesh Kumar
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  5. Vijayender Bhalla
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Corresponding author

Correspondence to Vijayender Bhalla.

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The authors declare that they have no competing interests.

Additional information

Pargat Singh and Ritika Gupta contributed equally to this paper.

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Singh, P., Gupta, R., Sinha, M. et al. MoS2 based digital response platform for aptamer based fluorescent detection of pathogens. Microchim Acta 183, 1501–1506 (2016). https://doi.org/10.1007/s00604-016-1762-2

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  • DOI: https://doi.org/10.1007/s00604-016-1762-2

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