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Optical enzyme-linked immunosorbent assay on a strip for detection of Salmonella typhimurium

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Abstract

A rapid and sensitive enzyme-linked immunosorbent assay (ELISA) on an immunochromatographic strip was evaluated for the measurement of Salmonella typhimurium. Two different optical signal generation methods (colorimetric and chemiluminescent) were used to develop the proposed immunostrip system. Colorimetric and chemiluminescent signals could be successfully generated using the proposed immunostrip system, and S. typhimurium could be quantitatively measured in the range of 9.2×103 to 9.2×106 CFU/mL within 20 min (colorimetric method) and 16 min (chemiluminescent method). The selectivity and feasibility of the proposed immunostrip was confirmed by conducting experiments with spiked samples using real river water.

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Authors and Affiliations

  1. Division of Chemical Engineering and Program of Bio-fusion Technology, Hankyong National University, Anseong, Gyeonggi-do, 456-749, Korea

    Sojung Park & Young-Kee Kim

  2. Department of Information & Control Engineering, Hankyong National University, Anseong, Gyeonggi-do, 456-749, Korea

    Yong Tae Kim

  3. Research Center of Chemical Technology, Hankyong National University, Anseong, Gyeonggi-do, 456-749, Korea

    Young-Kee Kim

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  1. Sojung Park
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  2. Yong Tae Kim
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Correspondence to Young-Kee Kim.

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Park, S., Kim, Y.T. & Kim, YK. Optical enzyme-linked immunosorbent assay on a strip for detection of Salmonella typhimurium . BioChip J 4, 110–116 (2010). https://doi.org/10.1007/s13206-010-4204-y

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  • DOI: https://doi.org/10.1007/s13206-010-4204-y

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