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Enzymatic sensing of glucose in artificial saliva using a flat electrode consisting of a nanocomposite prepared from reduced graphene oxide, chitosan, nafion and glucose oxidase

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Abstract

We report on the preparation of a nanoporous flat electrode by drop casting a nanocomposite consisting of reduced graphene oxide (rGO) and chitosan onto a polyester substrate. An underlying conductive surface is not required. The nanocomposite was characterized by scanning electron microscopy and electrochemical impedance spectroscopy. The 3D network of the composite was used as a scaffold for the immobilization of glucose oxidase (GOx). A well-defined signal related to direct GOx electrochemistry was registered and used to monitor levels of glucose. The resulting biosensor displays a linear response to glucose with a detection limit of 5 μM (at an S/N ratio of 3) and a sensitivity of 41.7 μA⋅mM−1∙cm−2. The sensor was applied to the determination of glucose in artificial saliva.

A 3rd generation amperometric glucose biosensor based on nanoporous electrode was prepared by drop casting reduced graphene oxide and chitosan composite onto a polyester substrate and integrated within an electrochemical cell formed by screen-printed reference and counter electrodes.

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Acknowledgments

Authors thank the Tunisian Ministry of Higher Education and Scientific Research (MHESR) and the University of Tunis El-Manar for the support given to AR and WA (research and travel funds).

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Authors and Affiliations

  1. Laboratoire de Chimie Analytique et Electrochimie (LR99ES15), Faculté des Sciences de Tunis, Université de Tunis El-Manar, Campus Universitaire Farhat Hached d’El-Manar, 2092, Tunis El-Manar, Tunisia

    Amal Rabti, Wicem Argoubi & Noureddine Raouafi

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  1. Amal Rabti
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  2. Wicem Argoubi
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  3. Noureddine Raouafi
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Correspondence to Noureddine Raouafi.

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Rabti, A., Argoubi, W. & Raouafi, N. Enzymatic sensing of glucose in artificial saliva using a flat electrode consisting of a nanocomposite prepared from reduced graphene oxide, chitosan, nafion and glucose oxidase. Microchim Acta 183, 1227–1233 (2016). https://doi.org/10.1007/s00604-016-1753-3

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  • DOI: https://doi.org/10.1007/s00604-016-1753-3

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