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Electrochemical immunoassay for the prostate specific antigen using a reduced graphene oxide functionalized with a high molecular-weight silk peptide

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Abstract

High molecular-weight silk peptide (SP) was used to functionalize the surface of nanosheets of reduced graphene oxide (rGO). The SP-rGO nanocomposite was then mixed with mouse anti-human prostate specific antigen monoclonal antibody (anti-PSA) and coated onto a glassy carbon electrode to fabricate an immunosensor. By using the hexacyanoferrate redox system as electroactive probe, the immunosensor was characterized by voltammetry and electrochemical impedance spectroscopy. The peak current, measured at the potential of 0.24 V (vs. SCE), is distinctly reduced after binding prostate specific antigen (PSA). Response (measured by differential pulse voltammetry) is linearly related to PSA concentration in the range from 0.1 to 5.0 ng · mL−1 and from 5.0 to 80.0 ng∙mL−1, and the detection limit is 53 pg∙mL−1 (at an SNR of 3). The immunosensor was successfully applied to the determination of PSA in clinical serum samples, and the results were found to agree well with those obtained with an enzyme-linked immunosorbent assay.

Nanosheets of reduced graphene oxide were functionalized with silk peptide and used to immobilize anti-PSA to fabricate an immunosensor for PSA.

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Acknowledgments

The authors gratefully acknowledge the financial supports from National Natural Science Foundation of China (No. 21275166), China Scholarship Council (No. 201307780006), and Research Foundation of General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China (No. 2013Qk286).

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan, 430074, China

    Yanying Wang, Ying Qu & Chunya Li

  2. Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China

    Yanying Wang & Kangbing Wu

  3. Chaozhou Quality and Measurement Supervision and Inspection Institute, Chaozhou, 521011, China

    Guishen Liu, Xiaodong Hou & Yina Huang

  4. The central hospital of Wuhan, Wuhan, 430014, China

    Wangze Wu

Authors
  1. Yanying Wang
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  2. Ying Qu
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  3. Guishen Liu
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  4. Xiaodong Hou
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  5. Yina Huang
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  6. Wangze Wu
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  7. Kangbing Wu
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  8. Chunya Li
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Correspondence to Kangbing Wu or Chunya Li.

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Wang, Y., Qu, Y., Liu, G. et al. Electrochemical immunoassay for the prostate specific antigen using a reduced graphene oxide functionalized with a high molecular-weight silk peptide. Microchim Acta 182, 2061–2067 (2015). https://doi.org/10.1007/s00604-015-1552-2

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  • DOI: https://doi.org/10.1007/s00604-015-1552-2

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