Skip to main content
SpringerLink
Log in

Microchip based and immunochromatographic strip assays for the visual detection of interleukin-6 and of tumor necrosis factor α using gold nanoparticles as labels

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

The enzyme-linked immunesorbent assay (ELISA) is most frequently used for the determination of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). However, this technique is time-consuming and requires a substantial sample volume. We have developed (a) a new kind of colloidal gold-based immunochromatographic strip, and (b) a colloidal gold-based immunochromatographic microchip for simultaneous, rapid, and visual detection of IL-6 and TNF-α. The lowest limit of detection is 62.5 ng mL−1 for IL-6, and 30 ng mL−1 for TNF-α. Both the strip method and the microchip method are highly specific, user-friendly, and can be read out without the need for instrumentation.

(a) a new kind of colloidal gold-based immunochromatographic strip, and (b) a colloidal gold-based immunochromatographic microchip were developed for the simultaneous, rapid, and visual detection of IL-6 and TNF-α. The lowest limit of detection is 62.5 ng mL−1 for IL-6, and 30 ng mL−1 for TNF-α.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Zhang XG, Klein B, Bataille R (1989) Interleukin-6 is a potent myeloma-cell growth factor in patients with aggressive multiple myeloma. Blood 74:11–13

    CAS  Google Scholar 

  2. Kishimoto T (1989) The biology of interleukin-6. Blood 74:1–10

    CAS  Google Scholar 

  3. Snick JV (1990) Interleukin-6: an overview. Annu Rev Immunol 8:253–278

    Article  Google Scholar 

  4. Ishihara K, Hirano T (2002) IL-6 in autoimmune disease and chronic inflammatory proliferative disease. Cytokine Growth Factor Rev 13:357–368

    Article  CAS  Google Scholar 

  5. Kishimoto T (2010) IL-6: from its discovery to clinical applications. Int Immunol 22:347–352

    Article  CAS  Google Scholar 

  6. Rincon M, Irvin CG (2012) Role of IL-6 in asthma and other inflammatory pulmonary diseases. Int J Biol Sci 8:1281–1290

    Article  CAS  Google Scholar 

  7. Naugler WE, Karin M (2008) The wolf in sheep’s clothing: the role of interleukin-6 in immunity, inflammation and cancer. Trends Mol Med 14:109–119

    Article  CAS  Google Scholar 

  8. Singh T, Newman AB (2011) Inflammatory markers in population studies of aging. Ageing Res Rev 10:319–329

    Article  CAS  Google Scholar 

  9. Helle M, Boeije L, Groot E, Vos A, Aarden L (1991) Sensitive ELISA for interleukin-6. Detection of IL-6 in biological fluids: synovial fluids and sera. J Immunol Methods 138:47–56

    Article  CAS  Google Scholar 

  10. Nuntaprasert A, Mori Y, Tsukiyama-Kohara K, Kai C (2005) Establishment of swine interleukin-6 sandwich ELISA. Comp Immunol Microbiol 28:121–130

    Article  CAS  Google Scholar 

  11. Ida N, Sakurai S, Hosaka T, Hosoi K, Kunitomo T, Matsuura Y, Kohase M (1990) An enzyme-linked immunosorbent assay for the measurement of human interleukin-6. J Immunol Methods 133:279–284

    Article  CAS  Google Scholar 

  12. Yücel A, Aybay C (2005) Development of ELISA systems for measurement of human tumor necrosis factor- alpha (TNF-a). Turk J Med Sci 35:385–393

    Google Scholar 

  13. Petyovka N, Lyach L, Voitenok NN (1995) Homologous ELISA for detection of oligomeric human TNF: properties of the assay. J Immunol Methods 186:161–170

    Article  CAS  Google Scholar 

  14. Yang H, Li D, He R, Guo Q, Wang K, Zhang X, Huang P, Cui D (2010) A novel quantum dots–based point of care test for syphilis. Nanoscale Res Lett 5:875–881

    Article  CAS  Google Scholar 

  15. Hou SY, Chen HK, Cheng HC, Huang CY (2007) Development of zeptomole and attomolar detection sensitivity of biotin-peptide using a dot-blot gold nanoparticle immunoassay. Anal Chem 79:980–985

    Article  CAS  Google Scholar 

  16. Chou SF (2013) Development of a manual self-assembled colloidal gold nanoparticle-immunochromatographic strip for rapid determination of human interferon-γ. Analyst 138:2620–2623

    Article  CAS  Google Scholar 

  17. Tripathi V, Nara S, Singh K, Singh H, Shrivastav TG (2012) A competitive immunochromatographic strip assay for 17-α-hydroxy progesterone using colloidal gold nanoparticles. Clin Chim Acta 413:262–268

    Article  CAS  Google Scholar 

  18. MacDougall D, Crummett WB (1980) Guidelines for data acquisition and data quality evaluation in environmental chemistry. Anal Chem 52:2242–2249

    Article  CAS  Google Scholar 

  19. Guo YR, Liu SY, Gui WJ, Zhu GN (2009) Gold immunochromatographic assay for simultaneous detection of carbofuran and triazophos in water samples. Anal Biochem 389:32–39

    Article  CAS  Google Scholar 

  20. Worsley GJ, Attree SL, Noble JE, Horgan AM (2012) Rapid duplex immunoassay for wound biomarkers at the point-of-care. Biosens Bioelectron 34:215–220

    Article  CAS  Google Scholar 

  21. Abe K, Hashimoto Y, Yatsushiro S, Yamamura S, Bando M, Hiroshima Y, Kido J, Tanaka M, Shinohara Y, Ooie T, Baba Y, Kataoka M (2013) Simultaneous immunoassay analysis of plasma IL-6 and TNF-a on a microchip. Plos One 8:e53620

    Article  CAS  Google Scholar 

  22. Frens G (1973) Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nat Phys 241:20–22

    Article  CAS  Google Scholar 

  23. He Y, Zhang S, Zhang X, Baloda M, Gurung A, Xu H, Zhang X, Liu G (2011) Ultrasensitive nucleic acid biosensor based on enzyme-gold nanoparticle dual label and lateral flow strip biosensor. Biosens Bioelectron 26:2018–2024

    Article  CAS  Google Scholar 

  24. Dungchai W, Siangproh W, Chaicumpa W, Tongtawe P, Chailapakul O (2008) Salmonella typhi determination using voltammetric amplification of nanoparticles: a highly sensitive strategy for metalloimmunoassay based on a copper-enhanced gold label. Talanta 77:727–732

    Article  CAS  Google Scholar 

  25. Choi DH, Lee SK, Oh YK, Bae BW, Lee SD, Kim S, Shin YB, Kim MG (2010) A dual gold nanoparticle conjugate-based lateral flow assay (LFA) method for the analysis of troponin I. Biosens Bioelectron 25:1999–2002

    Article  CAS  Google Scholar 

  26. Shyu RH, Shyu HF, Liu HW, Tang SS (2002) Colloidal gold-based immunochromatographic assay for detection of ricin. Toxicon 40:255–258

    Article  CAS  Google Scholar 

  27. Parolo C, Muñiz AE, Merkoçi A (2013) Enhanced lateral flow immunoassay using gold nanoparticles loaded with enzymes. Biosens Bioelectron 40:412–416

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Nature Science Foundation of China (21275019), the National Key Technology R&D Program of China (2009BAK59B01) and National Key Scientific Instrument and Equipment Development Projects of China (2012YQ040140).

Author information

Authors and Affiliations

  1. School of Life Science, Beijing Institute of Technology, Beijing 100081, China

    Yan Man, Xuefei Lv, Javed Iqbal, Guang Peng, Da Song, Congxiao Zhang & Yulin Deng

Authors
  1. Yan Man
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xuefei Lv
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Javed Iqbal
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Guang Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Da Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Congxiao Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yulin Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Xuefei Lv or Yulin Deng.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

(PDF 658 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Man, Y., Lv, X., Iqbal, J. et al. Microchip based and immunochromatographic strip assays for the visual detection of interleukin-6 and of tumor necrosis factor α using gold nanoparticles as labels. Microchim Acta 182, 597–604 (2015). https://doi.org/10.1007/s00604-014-1362-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00604-014-1362-y

Keywords

Search

Navigation