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Highly sensitive hybridization assay using the electrochemiluminescence of an ITO electrode, CdTe quantum dots functionalized with hierarchical nanoporous PtFe nanoparticles, and magnetic graphene nanosheets

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Abstract

We report on a disposable microdevice suitable for sandwich-type electrochemiluminescence (ECL) detection of DNA. The method is making use of CdTe quantum dots functionalized with hierarchical nanoporous PtFe (CdTe@PtFe) nanoparticles and with magnetic graphene nanosheets. The latter were selected as carriers for the capture DNA due to their excellent biomagnetic separation capability and electrical properties. The CdTe@PtFe nanoparticles were used to label the signal DNA which resulted in distinctly enhanced ECL owing to the large specific surface area and good electrical conductivity of the PtFe alloy. A DNA sensor was constructed on a disk-shaped indium tin oxide electrode that was fabricated via etching. Under optimal conditions, the biosensor responds linearly to DNA in the 0.02 fM to 5000 fM concentration range, with a detection limit as low as 15 aM. The electrode is regenerable. The method displays excellent specificity, extremely good sensitivity, and is highly reproducible.

CdTe quantum dots functionalized hierarchical nanoporous PtFe alloy (CdTe@PtFe) and magnetic graphene nanosheet (MGN) were applied for sensitive sandwich-type electrochemiluminescence DNA detection based on a disposable microdevice. The method displays excellent specificity, extremely good sensitivity, and is highly reproducible.

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Acknowledgments

This work was financially supported by Natural Science Research Foundation of China (21277058, 21175058, 21207048) and Natural Science Foundation of Shandong Province, China (ZR2011BQ019).

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Authors and Affiliations

  1. Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People’s.Republic of China

    Fang Liu, Wenping Deng, Yan Zhang, Jinghua Yu & Mei Yan

  2. Shandong Provincial Key Laboratory of Fluorine Chemistry and Chemical Materials, University of Jinan, Jinan, 250022, People’s Republic of China

    Shenguang Ge

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  1. Fang Liu
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  2. Wenping Deng
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  3. Yan Zhang
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  4. Shenguang Ge
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  5. Jinghua Yu
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  6. Mei Yan
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Correspondence to Mei Yan.

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Liu, F., Deng, W., Zhang, Y. et al. Highly sensitive hybridization assay using the electrochemiluminescence of an ITO electrode, CdTe quantum dots functionalized with hierarchical nanoporous PtFe nanoparticles, and magnetic graphene nanosheets. Microchim Acta 181, 213–222 (2014). https://doi.org/10.1007/s00604-013-1102-8

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  • DOI: https://doi.org/10.1007/s00604-013-1102-8

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