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Biotin-avidin-conjugated metal sulfide nanoclusters for simultaneous electrochemical immunoassay of tetracycline and chloramphenicol

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Abstract

We report on a protocol for a simultaneous competitive immunoassay for tetracycline (TC) and chloramphenicol (CAP) on the same sensing interface. Conjugates of TC and of CAP with bovine serum albumin were first co-immobilized on a glassy carbon electrode modified with gold nanoparticles. In parallel, monoclonal anti-TC and anti-CAP antibodies were conjugated onto CdS and PbS nanoclusters, respectively. In a typical assay, the immobilized haptens and the added target analytes competed for binding to the corresponding antibodies on the nanoclusters. Subsequently, Cd(II) and Pb(II) ions are released from the surface of the corresponding nanoclusters by treatment with acid and then were detected by square wave anodic stripping voltammetry. The currents at the peak potentials for Cd(II) and Pb(II) were used as the sensor signal for TC and CAP, respectively. This multiplex immunoassay enables the simultaneous determination of TC and CAP in a single run with dynamic ranges from 0.01 to 50 ng mL−1 for both analytes. The detection limits for TC and for CAP are 7.5 pg mL−1 and 5.4 pg mL−1, respectively. No obvious nonspecific adsorption and cross-reactivity was observed in a series of analyses. Intra-assay and inter-assay coefficients of variation were less than 10 %. The method was evaluated by analyzing TC and CAP in spiked samples of milk and honey. The recoveries range from 88 % to 107 % for TC, and from 91 % to 119 % for CAP.

We developed a new multiplexed electrochemical immunoassay for simultaneous determination of tetracycline and chloramphenicol, using metal sulfide nanoclusters as recognition elements.

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Acknowledgments

This work was financially supported by the National “973” Basic Research Program of China (2010CB732403), the National Natural Science Foundation of China (21075019, 41176079), the Research Fund for the Doctoral Program of Higher Education of China (20103514120003), the National Science Foundation of Fujian Province (2011 J06003), the China-Russia Bilateral Scientific Cooperation Research Program (NSFC/RFBR) (21211120157), and the Program for Changjiang Scholars and Innovative Research Team in University (IRT1116).

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  1. Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350108, People’s Republic of China

    Bingqian Liu, Bing Zhang, Guonan Chen & Dianping Tang

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  1. Bingqian Liu
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  2. Bing Zhang
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  3. Guonan Chen
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  4. Dianping Tang
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Correspondence to Dianping Tang.

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Liu, B., Zhang, B., Chen, G. et al. Biotin-avidin-conjugated metal sulfide nanoclusters for simultaneous electrochemical immunoassay of tetracycline and chloramphenicol. Microchim Acta 181, 257–262 (2014). https://doi.org/10.1007/s00604-013-1096-2

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