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NADH oxidation using modified electrodes based on lactate and glucose dehydrogenase entrapped between an electrocatalyst film and redox catalyst-modified polymers

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Abstract

Electrocatalytic NADH oxidation was investigated at an electrode architecture involving an electropolymerized layer of poly(methylene blue) (pMB) or poly(methylene green) (pMG) in combination with specifically designed toluidine blue or nile blue modified methacrylate-based electrodeposition polymers. Either NAD+-dependent lactate dehydrogenase or NAD+-dependent glucose dehydrogenase were entrapped between the primary electropolymerized layer of pMB or pMG and the methacrylate-based redox polymer. The composition of the polymer backbone and the polymer-bound redox dye was evaluated and it could be demonstrated that the combination of the electropolymerized pMB or pMG layer together with the dye modified methacrylate-based redox polymer shows superior properties as compared with either of the components alone. NADH was oxidized at an applied potential of 0 mV vs Ag/AgCl/KCl 3 M and current densities of 17 μA·cm−2 and 28 μA·cm−2 were obtained for modified electrodes based on lactate dehydrogenase and glucose dehydrogenase, respectively, at substrate saturation.

Schematic representation of the biosensor architecture based on a bottom layer of electropolymerized poly(methylene blue) (pMB) or poly(methylene green) (pMG) and a dehydrogenase (here LDH) entrapped within a layer of a specifically designed redox dye (here toluidine blue, TB) modified polymer.

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Acknowledgments

The authors are grateful to the EU for financial support in the framework of the project “3D-Nanobiodevice” (NMP4-SL-2009-229255) and to the German Science Foundation (DFG) in the framework of the project DFG - ERA (SCHU 929/10-1). E. A. is grateful for a fellowship provided by the Ministry of Higher Education and Scientific Research in Iraq covering her expenses during her stay in Germany.

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Authors and Affiliations

  1. Analytische Chemie – Elektroanalytik & Sensorik, Ruhr-Universität Bochum, Universitätsstr. 150, 44780, Bochum, Germany

    Eman Al-Jawadi, Sascha Pöller, Raoudha Haddad & Wolfgang Schuhmann

  2. Department of Chemistry, College of Science, Mosul University, Mosul, Iraq

    Eman Al-Jawadi

  3. Center for Electrochemical Sciences – CES, Ruhr-Universität Bochum, Universitätsstr. 150, 44780, Bochum, Germany

    Wolfgang Schuhmann

Authors
  1. Eman Al-Jawadi
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  2. Sascha Pöller
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  3. Raoudha Haddad
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  4. Wolfgang Schuhmann
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Corresponding author

Correspondence to Wolfgang Schuhmann.

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Al-Jawadi, E., Pöller, S., Haddad, R. et al. NADH oxidation using modified electrodes based on lactate and glucose dehydrogenase entrapped between an electrocatalyst film and redox catalyst-modified polymers. Microchim Acta 177, 405–410 (2012). https://doi.org/10.1007/s00604-012-0797-2

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  • DOI: https://doi.org/10.1007/s00604-012-0797-2

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