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ITO electrode modified by a gold ion implantation technique for direct electrocatalytic sensing of hydrogen peroxide

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Abstract

We report on a simple strategy for the fabrication of gold nanoparticles (AuNPs) on an indium tin oxide substrate using a modified ion implantation method. The morphology, structure and electrochemical features of AuNPs were characterized by atomic force microscopy, electrochemical impedance spectroscopy and cyclic voltammetry. The modified electrode has a large electrochemically active surface and enables strong loading with cytochrome c (Cyt c) proteins. It undergoes enhanced electron transfer at uncompromised electrochemical activity, and also displays good stability and repeatability. The immobilized Cyt c exhibits good electrocatalytic activity towards hydrogen peroxide (H2O2), with a linear relationship between the catalytic current during differential pulse voltammetry and the concentration of H2O2 in the 0.05 μM to 0.2 μM range. The detection limit (S/N = 3) is 0.01 μM.

A biosensor for H2O2 was developed by immobilizing cytochrome c on ITO electrode modified by gold ion implantation process. Ion implantation is a new type and an useful simply strategy to attach gold ions on the electrode. The biosensor exhibited a low detection limit, good reproducibility and stability, which indicated that ion-implanted gold nanoparticles could construct enzyme electrode for biosensor application.

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Acknowledgments

The authors wish to express their gratitude and appreciation for the financial support from the National Natural Science Foundation of China (No.20211130505) and the Key Laboratory of Beam Technology and Material Modification of Ministry of Education, China.

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Authors and Affiliations

  1. College of Chemistry, Beijing Normal University, Beijing, 100875, People’s Republic of China

    Fenfen Liang, Chenyao Liu, Jiao Jiao, Shuoqi Li & Jingbo Hu

  2. Key Laboratory of Beam Technology and Material Modification of Ministry of Education, Beijing Normal University, Beijing, 100875, People’s Republic of China

    Ji Xia

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  1. Fenfen Liang
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Correspondence to Jingbo Hu.

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Liang, F., Liu, C., Jiao, J. et al. ITO electrode modified by a gold ion implantation technique for direct electrocatalytic sensing of hydrogen peroxide. Microchim Acta 177, 389–395 (2012). https://doi.org/10.1007/s00604-012-0792-7

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  • DOI: https://doi.org/10.1007/s00604-012-0792-7

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