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SPR imaging biosensor for podoplanin: sensor development and application to biological materials

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Abstract

Podoplanin (PDP) is a small transmembrane protein and widely present in various specialized cells throughout the human body. It is a specific marker for identification of lymphatic vessel and a candidate marker for cancer stem cells in squamous cell carcinoma of the lung. We report on method for the highly selective determination of PDP by using a surface plasmon resonance imaging (SPRI) that exploits the highly selective interaction between PDP and anti-human PDP monoclonal antibody (IgG). The sensor has a dynamic range between 0.25 and 1.0 ng mL−1, and a detection limit of 15 pg mL−1. It was applied to the determination of PDP in blood plasma and tissue homogenates from paired normal and lung tumor tissue.

A novel method for the highly selective determination of podoplanin by using a Surface Plasmon Resonance Imaging (SPRI) technique has been developed. For sensor development, highly selective interaction between podoplanin and anti-human podoplanin monoclonal antibody (IgG) was applied.

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Authors and Affiliations

  1. Department of Electrochemistry, Institute of Chemistry, University of Bialystok, Al.J.Pilsudskiego11/4, PL-15-443, Bialystok, Poland

    Ewa Gorodkiewicz & Paulina Bajko

  2. Department of Clinical Molecular Biology, Medical University of Bialystok, Bialystok, Poland

    Radoslaw Charkiewicz & Jacek Niklinski

  3. Department of Clinical Pathology, Medical University of Bialystok, Bialystok, Poland

    Alicja Rakowska & Lech Chyczewski

Authors
  1. Ewa Gorodkiewicz
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  2. Radoslaw Charkiewicz
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  3. Alicja Rakowska
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  4. Paulina Bajko
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  5. Lech Chyczewski
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  6. Jacek Niklinski
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Correspondence to Ewa Gorodkiewicz.

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Gorodkiewicz, E., Charkiewicz, R., Rakowska, A. et al. SPR imaging biosensor for podoplanin: sensor development and application to biological materials. Microchim Acta 176, 337–343 (2012). https://doi.org/10.1007/s00604-011-0726-9

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  • DOI: https://doi.org/10.1007/s00604-011-0726-9

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