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Electrochemistry and biosensing activity of cytochrome c immobilized in macroporous materials

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Abstract

An amperometric biosensor for hydrogen peroxide (H2O2) has been constructed by immobilizing cytochrome c on an indium/tin oxide (ITO) electrode modified with a macroporous material. Cyclic voltammetry showed that the direct and quasi-reversible electron transfer of cytochrome c proceeds without the need for an electron mediator. A surface-controlled electron transfer process can be observed with an apparent heterogeneous electron-transfer rate constant (ks) of 29.2 s−1. The biosensor displays excellent electrocatalytic responses to the reduction of H2O2 to give amperometric responses that increase steadily with the concentration of H2O2 in the range from 5 μM to 2 mM. The detection limit is 0.61 μM at pH 7.4. The apparent Michaelis-Menten constant (Km) of the biosensor is 1.06 mM. This investigation not only provided a method for the direct electron transfer of cytochrome c on macroporous materials, but also established a feasible approach for durable and reliable detection of H2O2.

Biosensor for hydrogen peroxide was developed by immobilizing cytochrome c in the macroporous ordered silica foam (MOSF) through the electrostatic interaction. The achievement of the direct electron transfer between cytochrome c and electrode surface indicated that the MOSF modified electrode displayed good affinity and biocompatibility for cytochrome c.

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Acknowledgements

This work is supported by NSFC 20925517, STCSM (10XD1406000, 09JC1402600) and Shanghai Leading Academic Discipline B109.

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Authors and Affiliations

  1. Department of Chemistry and Key Lab of Molecular Engineering of Polymers of Chinese Ministry of Education, Fudan University, Shanghai, 200433, People’s Republic of China

    Ying Wang, Kai Guo, Jilie Kong & Baohong Liu

  2. ARC Centre of Excellence for Functional Nanomaterials and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, 4072, Australia

    Kun Qian & Chengzhong Yu

  3. IMAGES EA 4218 Centre de Phytopharmacie, Université de Perpignan, 52 Avenue Paul Alduy, F-66860, Perpignan Cedex, France

    Ying Wang & Jean-Louis Marty

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  1. Ying Wang
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Correspondence to Chengzhong Yu or Baohong Liu.

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Ying Wang and Kun Qian contributed equally to this work.

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Wang, Y., Qian, K., Guo, K. et al. Electrochemistry and biosensing activity of cytochrome c immobilized in macroporous materials. Microchim Acta 175, 87–95 (2011). https://doi.org/10.1007/s00604-011-0638-8

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