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Multilayer structured immunosensor based on a glassy carbon electrode modified with multi-wall carbon nanotubes, polythionine, and gold nanoparticles

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Abstract

An immunosensor for determination of salbutamol was developed. It based on glass carbon electrode (GCE) modified with a conductive multilayer film comprised of multi-wall carbon nanotubes, polythionine and gold nanoparticles. Salbutamol antibody was immobilized on the surface of the modified GCE which then was blocked with bovine serum albumin (BSA). The stepwise self-assembly process of the immunosensor was studied by cyclic voltammetry. The detection scheme is based on competitive binding of salbutamol to the sensor surface whose differential pulse voltammetric signal decreases after competitive binding of the salbutamol-BSA conjugate and free salbutamol to the salbutamol antibody. The sensor responds to salbutamol in 5 to 150 nM concentration range, with a detection limit of 1 nM. This method was applied to the precise and sensitive determination of salbutamol in spiked feed samples.

In this work, we constructed a salbutamol immunosensor which was based on salbutamol-Ab adsorbed on the AuNPs/PTH/MWCNTs/GCE. Just as the procedures shown in Graph 1, competitive immunoreaction was the experimental principle. The percentage of current response of the immunosensor was proportional to salbutamol concentrations in the range of 5–150 nM.

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Acknowledgments

The authors are grateful for the financial supports provided by the Technology Standard Project of Shanghai Science and Technology Commission (No. 10DZ0504000), the Nanometer Technology Special Project of Shanghai Science and Technology Commission (No. 0852nm06200) and (No. 1052nm06700).

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Authors and Affiliations

  1. Bor Luh Food Safety Center, Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Qin Mi, Zheng Wu Wang, Chun Yan Chai & Juan Zhang

  2. School of Chemical and Environmental Science, Nanjing Normal University, Nanjing, 210097, China

    Bo Zhao & Chang Yun Chen

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  1. Qin Mi
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  2. Zheng Wu Wang
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  3. Chun Yan Chai
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  4. Juan Zhang
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Correspondence to Zheng Wu Wang.

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Mi, Q., Wang, Z.W., Chai, C.Y. et al. Multilayer structured immunosensor based on a glassy carbon electrode modified with multi-wall carbon nanotubes, polythionine, and gold nanoparticles. Microchim Acta 173, 459–467 (2011). https://doi.org/10.1007/s00604-011-0572-9

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