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Integrated micro/nano-fluidic system for mixing and preconcentration of dissolved proteins

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Abstract

An integrated micro/nano-fluidic system is presented for protein analysis. It is comprised of an integrated micromixer (IMM) and a preconcentrator with a separation column. The passive and planar type of IMM is based on an unbalanced split and the cross collision of the fluidic streams. The IMM can be easily fabricated and integrated to the microfluidic system. The preconcentrator has nanochannels formed by the electrical breakdown of polydimethylsiloxane (PDMS) membrane by applying a high electrical shock, but without any nano-lithography. The integrated microdevice was used for sample preparation (mixing with tagging molecules) and subsequent concentration of proteins. Proteins were electrokinetically trapped near the junction of the micro/nanochannels. We show a conceptual design and a simple microfluidic system for purposes of mixing and preconcentration.

Mixing and preconcentration of dissolved proteins using an integrated micro/nano-fluidic system

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Acknowledgments

This research was supported by Agency for Defense Development Research Grant (Grant number: UD090078ID) and Basic Science Research Program through the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (KRF-2007-331-D00064 and 2009–0065065).

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Authors and Affiliations

  1. Department of Mechanical Engineering, Inha University, Incheon, 402–751, Republic of Korea

    Khalid Anwar, Taeheon Han, Samuel Yu & Sun Min Kim

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  1. Khalid Anwar
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  2. Taeheon Han
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  3. Samuel Yu
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  4. Sun Min Kim
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Correspondence to Sun Min Kim.

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Anwar, K., Han, T., Yu, S. et al. Integrated micro/nano-fluidic system for mixing and preconcentration of dissolved proteins. Microchim Acta 173, 331–335 (2011). https://doi.org/10.1007/s00604-011-0567-6

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  • DOI: https://doi.org/10.1007/s00604-011-0567-6

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