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Double layer enzyme modified carbon nanotubes as label for sandwich-type immunoassay of tumor markers

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Abstract

An immunosensor was prepared for the determination of carcinoembryonic antigen (CEA). It is based on the use of multiwalled carbon nanotubes (MWCNTs) along with horseradish peroxidase-labeled antibody. The enzyme was assembled onto MWCNTs templates using the layer-by-layer technique and then conjugated to carcinoembryonic secondary antibodies (Ab2) as the enzyme label. The resulting assembly results in a largely amplified sensitivity. The response is linear in the range of 0.05 to 45 ng mL-1, with a detection limit of 16.0 pg mL-1. The immunosensor possesses good stability and good reproducibility.

A new immunosensor with double-layer enzyme-modified carbon nanotubes as label for sandwich-type tumor markers was proposed in this study

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (No. 21075100), the Ministry of Education of China (Project 708073), the Natural Science Foundation of Chongqing City (NO. CSTC-2009BA1003), and High Technology Project Foundation of Southwest University (XSGX 02).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, Southwest University, Chongqing, 400715, People’s Republic of China

    Min Liang, Ruo Yuan, Yaqin Chai, Ligen Min & Zhongju Song

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  1. Min Liang
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  2. Ruo Yuan
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  3. Yaqin Chai
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  4. Ligen Min
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  5. Zhongju Song
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Correspondence to Ruo Yuan.

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Liang, M., Yuan, R., Chai, Y. et al. Double layer enzyme modified carbon nanotubes as label for sandwich-type immunoassay of tumor markers. Microchim Acta 172, 373–378 (2011). https://doi.org/10.1007/s00604-010-0502-2

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  • DOI: https://doi.org/10.1007/s00604-010-0502-2

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