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Tyrosinase biosensor based on a boron-doped diamond electrode modified with a polyaniline-poly(vinyl sulfonate) composite film

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Abstract

A BDD electrode modified with polyaniline (PANI) film doped with polyvinyl sulphonate (PVS), containing in-situ deposited tyrosinase (Tyr) enzyme was prepared and characterized. The PANI-PVS film was electrochemically polymerized onto the BDD surface by cyclic voltammetry (CV) at 50 mV.s−1 (versus Ag/AgCl). An increase in current density with increasing number of cycles was an indication of the polymer growth. The morphologies of the composite films were characterized by scanning electron microscopy (SEM). Electrochemical characterization confirmed the successful doping of the PANI film by PVS and that the template PVS directed the electron transfer at the biosensor interface. SEM provided evidence of the influence of the synthesis medium on the morphology and surface area of the composite film. Synthesis from HCl produced homogenous, finely granular thin film with increased surface area which is thought to be responsible for the increased redox currents measured. PANI/PVS served both as an efficient mediator and biocompatible enzyme immobilisation platform for the substrate L-tyrosine at low concentrations with apparent Michaelis-Menton (\( {\hbox{K}}_{\rm{m}}^{\rm{app}} \)) 1 × 10−2 µM.

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Authors and Affiliations

  1. Chemistry Department, University of the Western Cape, Private Bag X17, Bellville, 7535, South Africa

    Zelo Anatole Mangombo, Priscilla Baker, Emmanuel Iwuoha & David Key

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  1. Zelo Anatole Mangombo
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  2. Priscilla Baker
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  3. Emmanuel Iwuoha
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  4. David Key
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Correspondence to Priscilla Baker.

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Mangombo, Z.A., Baker, P., Iwuoha, E. et al. Tyrosinase biosensor based on a boron-doped diamond electrode modified with a polyaniline-poly(vinyl sulfonate) composite film. Microchim Acta 170, 267–273 (2010). https://doi.org/10.1007/s00604-010-0378-1

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  • DOI: https://doi.org/10.1007/s00604-010-0378-1

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