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Biofunctional magnetic nanoparticles as contrast agents for magnetic resonance imaging of pancreas cancer

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Abstract

We report on the fabrication and characterization of biofunctional magnetic nanoparticles as contrast agents for magnetic resonance imaging. The anti-cancer antigen 19-9 monoclonal antibody (a cancer-targeting antibody) was conjugated onto the magnetic contrast agents in an effort to detect pancreatic tumor. The structure, size, morphology and magnetic property of the biofunctional magnetic nanoparticles are characterized systematically by means of transmission electron microscopy and X-ray diffractometry. Furthermore, the interaction between the nanoparticles and pancreas cancers cells are investigated by atomic force microscope and transmission electron microscopy. Magnetic resonance imaging demonstrates that the conjugated nanoparticles can effectively target cancer cells both in vitro and in vivo, suggesting that they potentially can be used as contrast agents for magnetic resonance imaging of pancreas cancer.

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Acknowledgement

This work was supported by the LADP-SHNU (DZL806), National Nature Science Foundation of China (Nos. 20573075, 20773088), the National 863 Program(2006AA03Z319), the National Basic Research Program of China(2008CB617504), the Shanghai Science and Technology Committee (No. 07DZ22303 No. 0752 nm028 No. 06SU07003), and Shanghai Key Laboratory of Rare-earth Functional Materials (07dz22303). The authors thank Prof. M. Y. Gao for his assistance with MRI of nude mice.

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Authors and Affiliations

  1. Life and Environment Science College, Shanghai Normal University, Shanghai, 200234, People’s Republic of China

    Mingming Huang, Nengqin Jia & Hebai Shen

  2. Radiology Department, Ruijin Hospital, Shanghai, 200025, People’s Republic of China

    Zhongwei Qiao & Fei Miao

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  1. Mingming Huang
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  2. Zhongwei Qiao
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  3. Fei Miao
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  4. Nengqin Jia
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  5. Hebai Shen
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Correspondence to Hebai Shen.

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Huang, M., Qiao, Z., Miao, F. et al. Biofunctional magnetic nanoparticles as contrast agents for magnetic resonance imaging of pancreas cancer. Microchim Acta 167, 27 (2009). https://doi.org/10.1007/s00604-009-0210-y

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  • DOI: https://doi.org/10.1007/s00604-009-0210-y

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