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Albumin molecularly imprinted polymer prepared with a semi-rigid crosslinker in mixed organic/aqueous media

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Abstract

An approach using systematic component optimization for the formation of a molecularly imprinted polymer (MIP) has been adopted to imprint albumin. The result was a copolymer comprising 3-dimethylaminopropyl methacrylate and pentaerythritol tetraacrylate in a mole ratio of 1 to 11. Myoglobin was used in competitive re-binding experiments to compete with the polymer’s native template. The effects of monomer to crosslinker mole ratio, polymerization temperature and time were investigated. These results also showed that the MIP possessed a high selectivity and adsorption capacity with respect to albumin in competitive binding protocols when the interfering species was also present in solution. Polymerization temperature and time were all shown to have significant effects on the resulting albumin-MIP’s performance. Additionally, higher polymerization temperatures (> 38 °C) and extended polymerization times (> 48 h) increased monomer conversion as determined by HPLC, but decreased the selectivity and adsorption capacity of the MIP.

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References

  1. Du J, Shen L, Lu J (2003) Flow injection chemiluminescence determination of epinephrine using epinephrine-imprinted polymer as recognition material. Anal Chim Acta 489:183

    Article  CAS  Google Scholar 

  2. Hayden O, Bindeus R, Haderspo¨ck C, Mann K, Wirl B, Dickert FL (2003) Mass-sensitive detection of cells, viruses and enzymes with artificial receptors. Sens Actuators B 91:316

    Article  Google Scholar 

  3. Dickert FL, Hayden O, Halikias KP (2001) Synthetic receptors as sensor coatings for molecules and living cells. Analyst 126:766

    Article  CAS  Google Scholar 

  4. Ye L, Mosbach K (2001) Polymers recognizing biomolecules based on a combination of molecular imprinting and proximity scintillation: a new sensor concept. J Am Chem Soc 123:2901

    Article  CAS  Google Scholar 

  5. Liang C, Peng H, Bao X, Nie L, Yao S (1999) Study of a molecular imprinting polymer coated BAW bio-mimic sensor and its application to the determination of caffeine in human serum and urine. Analyst 124:1781

    Article  CAS  Google Scholar 

  6. Rezeli M, Kilár F, Hjertén S (2006) Monolithic beds of artificial gel antibodies. J Chromatogr A 1109:100

    Article  CAS  Google Scholar 

  7. Cheng Z, Zhang L, Li Y (2004) Synthesis of an enzyme-like imprinted polymer with the substrate as the template, and its catalytic properties under aqueous conditions. Chem Eur J 10:3555

    Article  CAS  Google Scholar 

  8. Takeuchi T, Mukawa T, Matsui J, Higashi M, Shimizu KD (2001) Molecularly imprinted polymers with metalloporphyrin-based molecular recognition sites coassembled with methacrylic acid. Anal Chem 73:3869

    Article  CAS  Google Scholar 

  9. McCluskey A, Holdsworth CI, Bowyer MC (2007) Molecularly imprinted polymers (MIPs): sensing, an explosive new opportunity? Org Biomol Chem 5:3233

    Article  CAS  Google Scholar 

  10. Fish WP, Ferreira J, Sheardy RD, Snow NH, O’Brien TP (2005) Rational design of an imprinted polymer: maximizing selectivity by optimizing the monomer-template ratio for a cinchonidine MIP, prior to polymerization, using microcalorimetry. J Liq Chromatogr Relat Technol 28:1

    Article  CAS  Google Scholar 

  11. Cooper AI, Hems WP, Holmes AB (1999) Synthesis of highly cross-linked polymers in supercritical carbon dioxide by heterogeneous polymerization. Macromolecules 32:2156

    Article  CAS  Google Scholar 

  12. Janiak DS, Kofinas P (2007) Molecular imprinting of peptides and proteins in aqueous media. Anal Bioanal Chem 389:399

    Article  CAS  Google Scholar 

  13. Lin HY, Rick J, Chou TC (2007) Optimizing the formulation of a myoglobin molecularly imprinted thin-film polymer—formed using a micro-contact imprinting method. Biosens Bioelectron 22:3293

    Article  CAS  Google Scholar 

  14. Hsu CY, Lin HY, Thomas JL, Wu BT, Chou TC (2006) Incorporation of styrene enhances recognition of ribonuclease A by molecularly imprinted polymers. Biosens Bioelectron 22:355

    Article  Google Scholar 

  15. Syu MJ, Nian YM, Chang YS, Lin XZ, Shiesh SC, Chou TC (2006) Ionic effect on the binding of bilirubin to the imprinted poly(methacrylic acid-co-ethylene glycol dimethylacrylate). J Chromatogr A 1122:54

    Article  CAS  Google Scholar 

  16. Ou SH, Wu MC, Chou TC, Liu CC (2004) Polyacrylamide gels with electrostatic functional groups for the molecular imprinting of lysozyme. Anal Chim Acta 504:163

    Article  CAS  Google Scholar 

  17. Hu CH, Chou TC (2009) Albumin molecularly imprinted polymer with high template affinity — prepared by systematic optimization in mixed organic/ aqueous media. Microchem J 91:53

    Article  CAS  Google Scholar 

  18. Xu Z, Liu L, Deng Q (2006) Study on the mechanism of binding specificity of metoclopramide-imprinted polymers. J Pharm Biomed Anal 41:701

    Article  CAS  Google Scholar 

  19. Zhu QZ, Haupt K, Knopp D, Niessner R (2002) Molecularly imprinted polymer for metsulfuron-methyl and its binding characteristics for sulfonylurea herbicides. Anal Chim Acta 468:217

    Article  CAS  Google Scholar 

  20. Idziak I, Benrebouh A, Deschamps F (2001) Simple NMR experiments as a means to predict the performance of an anti-17α-ethynylestradiol molecularly imprinted polymer. Anal Chim Acta 435:137

    Article  CAS  Google Scholar 

  21. Yu C, Mosbach K (2000) Influence of mobile phase composition and cross-linking density on the enantiomeric recognition properties of molecularly imprinted polymers. J Chromatogr A 888:63

    Article  CAS  Google Scholar 

  22. Piletsky SA, Piletska EV, Karim K, Freebairn KW, Legge CH, Turner APF (2002) Polymer cookery: influence of polymerization conditions on the performance of molecularly imprinted polymers. Macromolecules 35:7499

    Article  CAS  Google Scholar 

  23. Takeda K, Wada A, Yamamoto K, Moriyama Y, Aoki K (1989) Conformational change of bovine serum albumin by heat treatment. J Protein Chem 8:653

    Article  CAS  Google Scholar 

  24. Hart BR, Shea KJ (2002) Molecular imprinting for the recognition of N-Terminal Histidine peptides in aqueous solution. Macromolecules 35:6192

    Article  CAS  Google Scholar 

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Acknowledgement

The support of the National Cheng Kung University and Tatung University are gratefully acknowledged.

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Authors and Affiliations

  1. Department of Chemical Engineering, National Cheng Kung University, Tainan, 701, Taiwan

    Chih-Hsien Hu & Tse-Chuan Chou

  2. The Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan, 701, Taiwan

    Tse-Chuan Chou

  3. Department of Chemical Engineering, Tatung University, Taipei, 104, Taiwan

    Tse-Chuan Chou

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  1. Chih-Hsien Hu
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  2. Tse-Chuan Chou
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Correspondence to Tse-Chuan Chou.

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Hu, CH., Chou, TC. Albumin molecularly imprinted polymer prepared with a semi-rigid crosslinker in mixed organic/aqueous media. Microchim Acta 165, 399–405 (2009). https://doi.org/10.1007/s00604-009-0151-5

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  • DOI: https://doi.org/10.1007/s00604-009-0151-5

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