Abstract.
A method for the determination of trace amounts of enrofloxacin based on solid-phase spectrofluorimetry has been developed. The relative fluorescence intensity of enrofloxacin fixed on Sephadex SP C-25 gel was measured directly after packing the gel beads in a 1-mm silica cell, using a solid-phase attachment. The wavelengths of excitation and emission were 277 and 448 nm, respectively. The linear concentration range of application was 0.2–4.0 ng mL−1 of enrofloxacin, with a relative standard deviation of 1.2% (for a level of 2.0 ng mL−1) and a detection limit of 0.04 ng mL−1. The method was applied to the determination of enrofloxacin in commercial pharmaceutical formulations and spiked canine serum samples. It was validated using HPLC as a reference method and applying the standard addition methodology. Recovery levels of the method reached 100% in all cases.
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Ballesteros, O., Vílchez, J., Taoufiki, J. et al. Determination of the Antibacterial Drug Enrofloxacin by Solid-Phase Spectrofluorimetry. Microchim. Acta 148, 227–233 (2004). https://doi.org/10.1007/s00604-004-0265-8
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DOI: https://doi.org/10.1007/s00604-004-0265-8