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Allogeneic umbilical cord-derived mesenchymal stem cells for treating critical-sized bone defects: a translational study

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European Journal of Orthopaedic Surgery & Traumatology Aims and scope Submit manuscript

Abstract

Introduction

The current ‘gold-standard’ treatment of critical-sized bone defects (CSBDs) is autografts; however, they have drawbacks including lack of massive bone source donor site morbidity, incomplete remodeling, and the risk of infection. One potential treatment for treating CSBDs is bone marrow-derived mesenchymal stem cells (BM-MSCs). Previously, there were no studies regarding the use of human umbilical cord-mesenchymal stem cells (hUC-MSCs) for treating BDs. We aim to investigate the use of allogeneic hUC-MSCs for treating CSBDs.

Method

We included subjects who were diagnosed with non-union fracture with CSBDs who agreed to undergo hUC-MSCs implantation. All patients were given allogeneic hUC-MSCs. All MSCs were obtained and cultured using the multiple-harvest explant method. Subjects were evaluated functionally using the Lower Extremity Functional Scale (LEFS) and radiologically by volume defect reduction.

Result

A total of seven (3 male, 4 female) subjects were recruited for this study. The subjects age ranged from 14 to 62 years. All seven subjects had increased LEFS during the end of the follow-up period, indicating improved functional ability. The follow-up period ranged from 12 to 36 months. One subject had wound dehiscence and infection, and two subjects developed partial union.

Conclusion

Umbilical cord mesenchymal stem cells are a potential new treatment for CSBDs. Additional studies with larger samples and control groups are required to further investigate the safety and efficacy of umbilical cord-derived mesenchymal stem cells for treating CSBDs.

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Authors and Affiliations

  1. Department of Orthopaedic and Traumatology, Dr. Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

    Ismail Hadisoebroto Dilogo

  2. Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo Central Hospital, Faculty of Medicine, Universitas Indonesia, CMU 2 Building 5th Floor, Jl. Diponegoro 71, Jakarta Pusat, Indonesia

    Ismail Hadisoebroto Dilogo, Dina Rahmatika, Jeanne Adiwinata Pawitan, Isabella Kurnia Liem, Tri Kurniawati, Tera Kispa & Fajar Mujadid

  3. Department Histology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba 6, Jakarta, Indonesia

    Jeanne Adiwinata Pawitan

  4. Stem Cell and Tissue Engineering Research Center, IMERI, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

    Ismail Hadisoebroto Dilogo, Jeanne Adiwinata Pawitan, Isabella Kurnia Liem & Tri Kurniawati

  5. Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jl. Salemba 6, Jakarta, Indonesia

    Isabella Kurnia Liem

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  1. Ismail Hadisoebroto Dilogo
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  2. Dina Rahmatika
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Corresponding author

Correspondence to Ismail Hadisoebroto Dilogo.

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Conflict of interest

Ismail Hadisoebroto Dilogo, Dina Rahmatika, Jeanne Adiwinata Pawitan, Isabella Kurnia Liem, Tri Kurniawati, and Fajar Mujadid declare that they have no conflict of interest.

Ethical approval

Ethical approval was received from the Health Research Ethics Committee of Faculty of Medicine, Universitas Indonesia—Cipto Mangunkusumo Hospital, Jakarta, Indonesia. The Reference Number was 165/H2.F1/ETIK/2014.

Registration of research studies

Clinical Trial Registry Number: NCT 0172 5698.

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Dilogo, I.H., Rahmatika, D., Pawitan, J.A. et al. Allogeneic umbilical cord-derived mesenchymal stem cells for treating critical-sized bone defects: a translational study. Eur J Orthop Surg Traumatol 31, 265–273 (2021). https://doi.org/10.1007/s00590-020-02765-5

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  • DOI: https://doi.org/10.1007/s00590-020-02765-5

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