Abstract
Purpose
LDH caused by lumbar disc degeneration is associated with genetic factors. However, the role of ADAMTS6 and ADAMTS17 genes in LDH risk is still unknown.
Methods
To investigate the interaction between ADAMTS6 and ADAMTS17 variants in the susceptibility of LDH, five SNPs were genotyped in 509 patients and 510 healthy individuals. The experiment used logistic regression to calculate odds ratio (OR) and 95% confidence interval (CI). Multi-factor dimensionality reduction (MDR) was chosen to evaluate impact of interaction of SNP-SNP on susceptibility to LDH.
Results
ADAMTS17-rs4533267 is significantly associated with reducing risk of LDH (OR = 0.72, 95% CI = 0.57–0.90, p = 0.005). Stratified analysis indicates that ADAMTS17-rs4533267 is significantly associated with the reducing risk of LDH among participants aged ≤ 48 years old. In addition, we observed that ADAMTS6-rs2307121 was associated with increasing risk of LDH in females. MDR analysis shows that single-locus model composed by ADAMTS17-rs4533267 can be chosen as the best model for predicting susceptibility to LDH (CVC = 10/10, test accuracy = 0.543).
Conclusion
ADAMTS6-rs2307121 and ADAMTS17-rs4533267 are potentially associated with LDH susceptibility. In particular, ADAMTS17-rs4533267 has a strong association with reducing risk of LDH.
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Data availability
The datasets used or analysed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- LDH:
-
Lumbar disc herniation
- CI:
-
Confidence interval
- LBP:
-
Low back pain
- ADAMTS:
-
A disintegrin and metalloproteinase with thrombospondin motifs
- MRI:
-
Magnetic resonance imaging
- HWE:
-
Hardy–Weinberg equilibrium
- LDD:
-
Lumber intervertebral disc degeneration
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Acknowledgements
We are very grateful to the participants who provided blood samples for this experiment and the professionals responsible for blood collection, as well as to the reviewers and editors for their patience.
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HPB wrote the paper; JF analysed and interpreted the data; ZL conceived and designed the experiments; HPB and JF performed the experiments. All authors reviewed the manuscript.
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This study was performed in accordance with the World Medical Association Declaration of Helsinki and was approved by the ethics committee of the Second Affiliated Hospital of Xi'an Medical College. Written informed consent was obtained from all of the subjects before participating.
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Han, P., Jiang, F. & Zhang, L. The role of ADAMTS6 and ADAMTS17 polymorphisms in susceptibility to lumbar disc herniation in Chinese Han population. Eur Spine J 32, 1106–1114 (2023). https://doi.org/10.1007/s00586-023-07586-8
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DOI: https://doi.org/10.1007/s00586-023-07586-8