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Assessing evening primrose oil effects on the serum levels of omentin-1 and biochemical parameters in the diabetic rat model

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Abstract

As a complicated metabolic disease, diabetes mellitus causes irreparable microvascular and macrovascular complications for the affected person. In this regard, omentin-1 is an adipokine which is generally produced by omental fat tissue, and its levels are in connection with diabetes and its complications. In this study, the influence of evening primrose oil on the serum levels of omentin-1 and some of biochemical parameters in the diabetic rat model were investigated. In total, 24 healthy Wistar Albino male rats with a weight range of 200–250 g were classified into 4 groups of 6: First group: control normal, second group: EPO (evening primrose oil), third group: diabetic, fourth group: diabetic+EPO. After 20 days, serum samples were taken, and the required parameters were measured and analyzed. ELISA was utilized to determining serum omentin-1 levels. Also, the levels of biochemical factors TG, CHOL, HDL, LDL, FBS, and liver enzymes ALP, GGT, ALT, AST, and LDH were measured. There was a significant decrease in serum concentrations of omentin-1 in diabetic rats in comparison with the normal control group and EPO group. After using EPO, serum levels of omentin-1 increased in the diabetic+EPO group in comparison with the diabetic control group. Moreover, FBS and TG, ALP, ALT, and GGT were increased in diabetic rats. After using EPO, the levels of these parameters decreased significantly in the diabetic+EPO group compared to the diabetic control group. Based on the results, it can be concluded that EPO can be a potential supplement to control diabetes and reduce blood sugar and diabetes-induced complications.

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Acknowledgements

F Kh, M Z, and P R worked on the conception and design, collection and assembly of data, manuscript writing, and data analysis and interpretation. P R and A G worked on the data analysis and interpretation. A G and M Z wrote the paper. All authors approved the manuscript.

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Authors and Affiliations

  1. Department of Clinical Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

    Mahsa Zebardast, Fatemeh Kheradmand, Parviz Ranjbarvan & Ali Golchin

  2. Cellular and Molecular Research Center, Cellular and Molecular Research Institute, Urmia University of Medical Sciences, Urmia, Iran

    Fatemeh Kheradmand, Parviz Ranjbarvan & Ali Golchin

  3. Solid Tumor Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran

    Fatemeh Kheradmand

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  1. Mahsa Zebardast
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  2. Fatemeh Kheradmand
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  3. Parviz Ranjbarvan
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  4. Ali Golchin
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Correspondence to Fatemeh Kheradmand.

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This work was supported by Urmia University of Medical Sciences, grant no. 11454.

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The authors declare that they have no conflict of interest.

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This study was approved by Urmia University of Medical Sciences Committee for Experiments Using Animals (approval no. IR.UMSU.AEC.1401.005) based on the Fundamental Guidelines for Proper Conduct of Animal Experiment and Related Activities in Academic Research Institutions.

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Zebardast, M., Kheradmand, F., Ranjbarvan, P. et al. Assessing evening primrose oil effects on the serum levels of omentin-1 and biochemical parameters in the diabetic rat model. Comp Clin Pathol 33, 193–199 (2024). https://doi.org/10.1007/s00580-023-03538-2

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