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Quantification of liver lipid deposition and melano-macrophages in lesser guitarfish Zapteryx brevirostris submitted to different feeding cycles

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Abstract

The objective of this study was to evaluate and quantify the fat storage in the liver of lesser guitarfish Zapteryx brevirostris submitted to different periods of restriction and refeeding. In a water recirculation system, 21 animals were divided into three groups: control (n=7), fed every day; W1 (n=7), with one week of restriction and two of refeeding and W2 (n=7), with two weeks of restriction and two of refeeding. After 76 days, the animals were euthanized and liver samples were collected, paraffin embedded, stained with PAS and evaluated with stereological procedures and histopatological changes were recorded. The area occupied by fat in the liver of control group was higher than that found in groups W1 and W2 (p<0.001). Melano-macrophage center areas and diameters have risen with increased fasting period while there were swollen and necrotic hepatocytes. These results pointed to physiological adaptations and liver lesions caused by free radicals during fasting periods. Similarly to teleost fish, there was a consumption of lipid reserves while enhancing the gluconeogenesis during fasting.

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Authors and Affiliations

  1. AQUAM - Production and Conservation of Biodiversity of Aquatic Species, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 7712, Porto Alegre, RS, 91540-000, Brazil

    Iuri Moraes Neyrão

  2. School of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87 - Cidade Universitária, São Paulo, SP, 05508-270, Brazil

    André Luiz Veiga Conrado & Carlos Eduardo Malavasi Bruno

  3. Rua Professor Joaquim Lauro Monte Claro Neto, São Paulo State Fisheries Institute, 2275, Praia de Itaguá, Ubatuba, SP, 11680-000, Brazil

    Verônica Takatsuka & Venâncio Guedes de Azevedo

Authors
  1. Iuri Moraes Neyrão
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  2. André Luiz Veiga Conrado
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  3. Verônica Takatsuka
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  4. Carlos Eduardo Malavasi Bruno
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  5. Venâncio Guedes de Azevedo
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Correspondence to André Luiz Veiga Conrado.

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Conflict of interest

Iuri Moraes Neyrão declares that he has no conflict of interest.

André Luiz Veiga Conrado declares that he has no conflict of interest.

Verônica Takatsuka declares she has no conflict of interest.

Carlos Eduardo Malavasi Bruno declares he has no conflict of interest.

Venâncio Guedes de Azevedo declares he has no conflict of interest.

Ethical approval

All applicable international, national and/or institutional guidelines for the care and use of animals were followed.

This research is in accordance with the ethical principles of animal experimentation adopted by the Brazilian Animal Experimentation College (COBEA) and was approved by the Committee of Ethics on Animal Experimentation of the São Paulo State Fisheries Institute, São Paulo, Brazil # 12/2016 with license of the Brazilian Institute of Environment and Renewable Natural Resources, IBAMA # 49980-3/2016.

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Neyrão, I.M., Conrado, A.L.V., Takatsuka, V. et al. Quantification of liver lipid deposition and melano-macrophages in lesser guitarfish Zapteryx brevirostris submitted to different feeding cycles. Comp Clin Pathol 28, 805–810 (2019). https://doi.org/10.1007/s00580-019-02953-8

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  • DOI: https://doi.org/10.1007/s00580-019-02953-8

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