Skip to main content
SpringerLink
Log in

Comparative study of oxidative status in blood of asthmatic patients

  • Original Article
  • Published:
Comparative Clinical Pathology Aims and scope Submit manuscript

Abstract

Bronchial asthma (BA) is a chronic inflammatory lungs disease, resulting in an airflow restriction, hyperactivity, and airway remodeling. The aim of present study is to investigate and compare the oxidative stress levels in blood of asthmatic patients differ in the disease control degree. In the current study were included 30 patients with bronchial asthma and 24 healthy volunteers. Patients were diagnosed with BA with allergic component longer than 1 year. For this purpose were explored the reactive oxygen species (ROS) and reactive nitrogen species (RNS), final products of lipids and proteins. Also, were studied the relationship between oxidative stress parameters and C-reactive protein (CRP) as a marker of inflammation degree. By using the electron paramagnetic resonance (EPR) spin trapping technique, ongoing the real-time oxidative processes were confirmed in blood samples isolated from asthmatic patients differing in the disease control degree. Moreover, positive correlation was found between the levels of studied oxidative stress (OS) biomarkers and CRP as a marker of inflammation degree. The oxidative processes in real time were demonstrated in BA patients. The correlation analysis results confirm the development and maintenance of inflammatory processes in respiratory tract is associated with the oxidative and nitrosative stress.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Allegra L, Braga PC, Dal Negro R (2012) Methods in Asthmology. Springer Science and Business Media eds

    Google Scholar 

  • Barrera G (2012) Oxidative stress and lipid peroxidation products in Cancer progression and therapy, review article. Intern Scholarly Res Network ISRN Oncology 21

  • Cantin AM, Hartl D, Konstan MW, Chmiel JF (2015) Inflammation in cystic fibrosis lung disease: pathogenesis and therapy. J Cystic Fibrosis 14(4):419–430

    Article  CAS  Google Scholar 

  • Cho YS, Moon HB (2010) The role of oxidative stress in the pathogenesis of asthma. Allergy, Asthma Imm Res 2(3):183–187

    Article  CAS  Google Scholar 

  • Dimov D, Kurzawski M, Tacheva T et al (2013a) IL1RN allele 3 as a protective factor in bronchial asthma in Bulgarian population, allergy, asthma & immunophys.: From Basic Sc to Clin Manag

  • Dimov D, Tacheva T, Ilieva V et al (2013b) Investigation of GSTM1 and GSTT1 null genotypes as risk factors for Bronchial asthma in a population of central Bulgaria. Allergy, asthma & immunophys.: From Basic Sc to Clin Manag 17

  • Dix TA, Aikens J (1993) Mechanisms and biological relevance of lipid peroxidation initiation. Chem Res in Toxicol 6(1):2–18

    Article  CAS  Google Scholar 

  • Folkerts G, Nijkamp FP (2006) Nitric oxide in asthma therapy. Curr Pharm Des 12(25):3221–3232

    Article  PubMed  CAS  Google Scholar 

  • Gamon LF, Wille U (2016) Oxidative damage of biomolecules by the environmental pollutants NO2• and NO3•. Accounts of Chem Res 49(10):2136–2145

    Article  CAS  Google Scholar 

  • Global Initiative for Asthma (GINA) (2006) revision www.ginasthma.com

  • Halliwell B, Gutteridge JM (2015) Free radicals in biology and medicine. Oxford Univ Press, USA

    Book  Google Scholar 

  • Hassan SH, Rehman J, Ashfaq A (2015) Bronchial asthma; duration of asthma is not correlated with total anti-oxidant capacity and lung function parameters. Prof Med J 22(11)

  • Kharitonov SA, Yates D, Robbins RA, Barnes PJ, Logan-Sinclair R, Shinebourne EA (1994) Increased nitric oxide in exhaled air of asthmatic patients. Lancet 343:133–135

    Article  PubMed  CAS  Google Scholar 

  • Khuseyinova N, Imhof A, Trischler G (2003) Determination of C-reactive protein: comparison of three high-sensitivity immunoassays. Clin Chem 49(10):1691–1695

    Article  PubMed  CAS  Google Scholar 

  • Kirkham P, Rahman I (2006) Oxidative stress in asthma and COPD: antioxidants as a therapeutic strategy. Pharmacol Ther 11:476–494

    Article  CAS  Google Scholar 

  • Kovarik KR, Kovarik JE (2016) Method and system for targeting the microbiome to promote health and treat allergic and inflammatory diseases. U.S. Patent 9:457–477

    Article  Google Scholar 

  • Levine SJ (1995) Bronchial epithelial cell-cytokine interactions in airway inflammation. J Investig Med 43:241–249

    PubMed  CAS  Google Scholar 

  • Nadeem A, Chhabra SK, Masood A (2003) Increased oxidative stress and altered levels of antioxidants in asthma. J Allergy Clin Immunol 111 (1): 72, 78

  • Palmieri B, Sblendorio V (2007) Oxidative stress tests: overview on reliability and use. Europ rev for med and pharmacolog sci 11(6):383–399

    CAS  Google Scholar 

  • Pandey R, Singh M, Singhal U et al (2013) Oxidative/nitrosative stress and the pathobiology of chronic obstructive pulmonary disease. J Clin Diagn Res (JCDR) 7(3):580

    Google Scholar 

  • Plaser ZA, Cushman LL, Jonson BC (1966) Estimation of product of lipid peroxidation (Malonyl Dialdehyde) in biochemical systems. Anal Biochem 16:359–364

    Article  Google Scholar 

  • Shi H, Sui Y, Wang X et al (2005) Comparative biochemistry and physiology part C: toxicology & pharmacol 140 (1): 115–121

  • Spasojević I, Mojović M, Ignjatović A et al (2011) The role of EPR spectroscopy in studies of the oxidative status of biological systems and the antioxidative properties of various compounds. J Serb Chem Soc 76(5):647–677

    Article  CAS  Google Scholar 

  • Stadtman ER, Levine RL (2000) Protein oxidation. Ann N.Y. Acad Sci 899:191–208

    Article  PubMed  CAS  Google Scholar 

  • Suzuki YJ, Carini M, Butterfield DA (2010) rotein carbamoylation. Antioxid Redox Signal 12:323–325

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Taylor DR (2012) Biomarkers of inflammation in asthma: a clinical perspective. In Seminars in respiratory and critical care medicine, Thieme Med Publish 33 (06): 620–629

  • Winczura A, Zdżalik D, Tudek B (2012) Damage of DNA and proteins by major lipid peroxidation products in genome stability. Free Rad Res 46(4):442–459

    Article  CAS  Google Scholar 

  • Wong CM, Bansal G, Marcocci L, Suzuki YJ (2012) Proposed role of primary protein carbonylation in cell signaling. Redox Rep 17(2):90–94

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Wood LG, Gibson PG, Garg ML (2003) Biomarkers of lipid peroxidation, airway inflammation and asthma. Eur Respir J 21:177–186

    Article  PubMed  CAS  Google Scholar 

  • Yokoyama K, Hashiba K, Wakabayashi H (2004) Inhibition of LPS-stimulated NO production in mouse macrophage-like cells by Tropolones. Anticans Res 24:3917–3922

    CAS  Google Scholar 

  • Yoshioka T, Iwamoto N, Lto K (1996) An application of electron paramagnetic resonance to evaluate nitric oxide and its quenchers. J Am Soci Nephrol 7:961–965

    CAS  Google Scholar 

  • Zuo L, Koozechian MS, Chen LL (2014) Characterization of reactive nitrogen species in allergic asthma. Ann Allergy Asthma Immunol 112(1):18–22

    Article  PubMed  CAS  Google Scholar 

  • Zuo L, Otenbaker NP, Rose BA, Salisbury KS (2013) Molecular mechanisms of reactive oxygen species-related pulmonary inflammation and asthma. Mol Immunol 56(1):57–63

    Article  PubMed  CAS  Google Scholar 

Download references

Funding

This study was funded by University scientific project № 19/2015 of Medical faculty, Trakia University, Stara Zagora, Bulgaria.

Author information

Authors and Affiliations

  1. Department Chemistry and Biochemistry, Medical Faculty, Trakia University, 11 Armeiska str., 6000, Stara Zagora, Bulgaria

    Galina Nikolova, Yanka Karamalakova, Antoaneta Zheleva & Veselina Gadjeva

  2. Department of Internal Medicine, Medical Faculty, University Hospital, Trakia University, Stara Zagora, Bulgaria

    Vanya Ilieva

Authors
  1. Galina Nikolova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Vanya Ilieva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yanka Karamalakova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Antoaneta Zheleva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Veselina Gadjeva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Galina Nikolova.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This article does not contain any studies with animals performed by any of the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Nikolova, G., Ilieva, V., Karamalakova, Y. et al. Comparative study of oxidative status in blood of asthmatic patients. Comp Clin Pathol 27, 1057–1063 (2018). https://doi.org/10.1007/s00580-018-2701-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00580-018-2701-x

Keywords

Search

Navigation